Characterization and grouping of Trypanosoma cruzi stocks by DNA base-specific fluorochromes and discriminant analysis.

Fluorochromes with G-C and A-T specificity were used for a single-cell DNA analysis of the blood-stream forms of 14 Trypanosoma cruzi stocks in a cytofluorometric assay. The kinetoplast contained 22.3%-37.

Characterization and grouping of Trypanosoma brucei brucei, T.b. gambiense and T.b. rhodesiense by quantitative DNA-cytofluorometry and discriminant analysis.

For characterization and differentiation 13 stocks of Trypanosoma brucei spp. were used for a quantitative cytofluorometric determination of their DNA fluorescence intensities by two base-pair-specific fluorochromes. T.

Cytofluorometry as a method for the differentiation of trypanosomes.

The DNA binding guanine specific antibiotic, chromomycin A3, has been evaluated for fluorescence intensity measurements of T. cruzi, T. brucei brucei and T.

Comparative characterization of Venezuelan Trypanosoma cruzi stocks by electron microscopy, isoelectrofocusing and lectin typing.

The intraspecific variation among culture forms of 14 Venezuelan Trypanosoma cruzi stocks were examined by kDNA configuration, isoenzymes, and agglutination behaviour of lectins. The results have shown that in all of the stocks the central band of kDNA is present, showing that the stocks are parasites of the subgenus Schizotrypanum. By isoenzymes and lectin typing it has been found that the stocks belong to the isoenzyme group I and the lectin-type PNA which were already described for other Venezuelan stocks.

The occurrence of N-acetyl- and N-glycoloylneuraminic acid in Trypanosoma cruzi.

Different strain of Trypanosoma cruzi were analysed to have 65--105 micrograms of sialic acids per 10(10) cells. By thin-layer chromatography, and in part by gas liquid chromatography and gas-liquid chromatography-mass spectrometry, all strains were found to contain N-acetyl- and N-glycoloylneuraminic acid in various ratios. After incubation of the parasites with either [3H]acetate or N-acetyl-[3H]mannosamine, no radioactivity was found in the sialic acids, thus leading to the suggestion that the parasites are unable to synthesize sialic acids from their precursors.

[Comparative electron microscope studies on the kinetoplast morphology of bat-trypanosomes and Trypanosoma cruzi (author's transl)].

Comparative studies on the ultrastructural morphology of kinetoplasts of trypanosomes belonging to the subgenus Schizotrypanum have been made. Three isolates of Trypanosoma vespertilionis and two strains of Trypanosoma dionisii derived from European bats were tested. Comparison was made also with two isolates from Brasilian bats characterized as T.

Leishmania donovani: ultrastructural localization of diaminobenzidine reactivity in the amastigotes.

Intracellular amastigotes of Leishmania donovani obtained from spleens of infected hamsters were studied by means of the diaminobenzidine technique for the presence of cytochromes and the activities of cytochrome oxidase and peroxidase. In the absence of H2O2, the oxidation of DAB, evidenced by electron-dense deposits localized on the cristae, inclusions, and enveloping membranes of the mitochondria and kinetoplast, revealed the activity of the cytochrome oxidase and the presence of the cytochromes. The increased deposition of DAB oxidation especially on the enveloping membranes in the presence of H2O2 suggests the activity of a peroxidase, probably cytochrome c peroxidase.

Protein typing by disc electrophoresis of some species of trypanosomes with special emphasis to Trypanosoma cruzi.

Proteins extracted from culture forms of T. cruzi, T. cruzi-like strains, T.

Ultrastructural localization of diaminobenzidine reactivity in leishmania donovani promastigotes.

The ultrastructural localization of the activities of two enzyme systems in the culture forms of Leishmania donovani was shown by means of the diaminobenzidine techniques. The consistent deposition of electron dense reaction product of DAB oxidation without H2O2 in the kinetoplast and mitochondrial cristae and membranes was taken as evidence of the presence of cytochrome oxidase activity and cytochrome c. In the presence of H2O2, a more intense DAB oxidation was attributed to the activity of a peroxidase, possibly cytochrome c peroxidase.

[Agglutination reaction of T. cruzi, T. cruzi like Strains, T. rangeli and T. conorhini with Soja hispida lectin and Aaptos papillata protectin (author's transl)].

Protectin from the sponge Aaptos papillata (Keller) was used in the characterization of five strains of T. cruzi (Venezuela, Guatemala, Y. Brasilien, Peru, Wien) and six T.

[Strain related differences in the immunodiagnosis of malaria (author's transl)].

In infections with P. falciparum and P. vivax antibody titers were found to differ in relation to strains.

[Comparative electron microscope studies on the labelling Leishmania donovani, Leishmania tropica and Leishmania braziliensis with ferritin (author's transl)].

The labelling of negative charges on the cell surface of the developing promastigote forms in Leishmania donovani, L. tropica and L. braziliensis was determined using cationized ferritin and electron microscopy.

[Comparative morphological studies on the kinetoplast of various species of trypanosomes, with special reference to trypanosoma cruzi (author's transl)].

Comparative electron microscope studies on the morphology of the kinetoplast DNA (K-DNA) of the epimastigotes in many trypanosome species were carried out under standardized conditions. The K-DNA shows a morphological variation during the cell cycle of culture forms of the trypanosome species under study. In longitudinal sections of the kinetoplast, the K-DNA of T.

[An electron microscopic study on a strain of Trypanosoma vivax maintained in cattle (author's transl)].

The ultrastructure of the blood forms of Trypanosoma vivax from its natural host, cattle, is described. The pellicula, consisting of an unit membrane with a superimposed surface coat, the structure and attachment of the flagellum and the sub-pellicular microtubules showed the usual structural and organizational features. Cell organelles and cytoplasmic inclusions such as cell nucleus, mitochondria, kinetoplast, Golgi-complex, endoplasmic reticulum and membrane-isolated vacuoles, which occur in trypanosomidae, are presented and described.