Bacterial renal infection in rats produced by direct injection of bacteria into renal tissue--an experimental model.

A new method for producing an experimental model of renal infection in rats is described. It is based on a direct injection of a concentrated E. coli suspension either into both kidneys or only one of them.

Comparison of fluorescent-antibody methods and enrichment serology for the detection of Salmonella.

Four rapid methods for detection of Salmonella, (i) the conventional fluorescent-antibody (FA) technique, (ii) a rapid direct FA technique, (iii) microcolony FA, and (iv) enrichment serology (ES), were compared with conventional cultural procedures. A total of 347 subsamples representing 16 different food prototypes, alleged to be naturally contaminated with Salmonella, were analyzed. From these samples, 52 were found to contain Salmonella by cultural methods.

Control of synthesis de novo of ascorbate oxidase in the mustard seedling (Sinapis alba L.) by phytochrome.

Density-labelling with deuterium oxide has been used to distinguish preexisting from newly made ascorbate oxidase (EC 1.10.3.

Phytochrome-mediated induction of ascorbate oxidase in different organs of a dicotyledonous seedling (Sinapis alba L.).

The time courses of the level of ascorbate oxidase (AO; EC 1.10.3.

Accumulation of protochlorophyll and chlorophyll a as controlled by photomorphogenically effective light.

Data are presented which indicate that the rate of synthesis and the pool size of photoconvertible protochlorophyll(ide) in the cotyledons of the mustard seedling are controlled by the active form of phytochrome (Pfr). Inductionreversion experiments show that formation of chlorophyll a through photoconversion of the protochlorophyll(ide) by repeated red pulses (5 min each) has no effect on synthesis of carotenoids and galactolipids. Since the protochlorophyll(ide)-converting activity of the standard far-red light used in this laboratory is very low, chlorophyll-a accumulation is very slow under continuous standard far-red light.

Phytochrome-mediated growth responses in green and etiolated Lemna minor.

The growth of Lemna minor in darkness is log-linear, at a much reduced rate compared to growth in white or red light. This rate of frond production in darkness is stimulated by kinetin, yeast extract, and thiamine either in green plants transferred directly from the light or in plants which had been grown in the dark for 54 days. (Fig.