Cell-free translation of soil-borne wheat mosaic virus RNAs.

The genome of soil-borne wheat mosaic (SBWMV) virus appears to be composed of two RNAs. Three strains have the same large RNA, designated 1.0L RNA, but differ in the size of the smaller RNA, designated 0.

Complexity of the Argentina mild strain of barley stripe mosaic virus.

Barley stripe mosaic virus (BSMV) is known to have unusual strain-specific variations in the number of high molecular weight RNAs that can be resolved by gel electrophoresis. Analysis with recombinant DNA clones has revealed that all strains contain three distinct hybridizing species of RNA, which we now designate alpha, beta and y. The alpha- and beta-RNAs are about 4200 and 3650 nucleotides (NT), respectively, but the gamma-RNAs vary in size depending on the strain.

Recessive alleles found at R and C loci in maize stocks showing aberrant ratio at the A locus.

Corn stocks showing virus-induced aberrant ratio (AR) at the "A" locus were found to have recessive alleles at the R and/or C loci. Since by the known pedigree these loci should be homozygous dominant, the results suggest an inactivation of maize genes by a mechanism as yet unknown. The presence of recessive alleles at these additional loci can explain the segregation ratios obtained in these particular stocks.

Ultraviolet absorption spectra and difference spectra of barley stripe mosaic and tobacco mosaic viruses in buffer and sodium dodecyl sulfate.

Ultraviolet absorption spectra and difference spectra were determined for barley stripe mosaic virus (BSMV) virions and protein and for tobacco mosaic virus (TMV) virions and protein in buffer and in sodium dodecyl sulfate (SDS). The absorbance at 260 nm of BSMV virions disrupted in SDS was 92% of that of intact virions after correction for light scattering. The corresponding value for TMV was 76%.

Evidence for gene inactivation in the virus-induced aberrant ratio phenomenon in maize.

Reciprocal crosses were made between pairs of plants showing the "aberrant ratio" (AR) effect at the a locus, and simultaneously between these plants and an aa tester. The results suggested that the AR effect in the particular line that was used could be explained by inactivation of a gene other than A, but also required for aleurone color. Segregation ratios in additional crosses largely fit expectations predicted on the basis of this hypothesis.

Differentiation of legume carlaviruses based on their biochemical properties.

Nucleic acids of alfalfa latent virus (ALV), pea streak virus (PSV), and red clover vein mosaic virus (RCVMV) were hydrolyzed by pancreatic RNase but not by DNase. ALV and PSV each had one single-stranded RNA of 38.0 and 37.