GALNT2 enhances migration and invasion of oral squamous cell carcinoma by regulating EGFR glycosylation and activity.

Objectives: Oral squamous cell carcinoma (OSCC) is one of the leading cancers worldwide. Aberrant glycosylation affects many cellular properties in cancers, including OSCC. This study aimed to explore the role of N-acetylgalactosaminyltransferase 2 (GALNT2) in OSCC.

Fast-track improves post-operative nutrition and outcomes of colorectal surgery: a single-center prospective trial in China.

Fast-track (FT) has been shown to enhance post-operative recovery. The aim of this study was to compare the effects of FT and traditional nutrition on post-operative rehabilitation, as well as evaluate the feasibility of applying FT in nutrition management of colorectal surgery. A prospective and randomized controlled trial was performed.

Elbow joint fatigue and bench-press training.

Context: Bench-press exercises are among the most common form of training exercise for the upper extremity because they yield a notable improvement in both muscle strength and muscle endurance. The literature contains various investigations into the effects of different bench-press positions on the degree of muscle activation. However, the effects of fatigue on the muscular performance and kinetics of the elbow joint are not understood fully.

[Development and assessment of Schistosoma japonicum antibody test kit (IHA)].

Objective: To develop a Schistosoma japonicum antibody test kit (IHA).

Methods: According to the requirement of Good Manufacturing Practice (GMP) and Quality Control (QC) of drugs, the components of kit as well as its preparation method and procedure were studied, and the test kit was assembled and its diagnostic effect was assessed.

Results: The sensitivity and specificity were up to 94.

Conductance of tailored molecular segments: a rudimentary assessment by Landauer formulation.

One of the strengths of molecular electronics is the synthetic ability of tuning the electric properties by the derivatization and reshaping of the functional moieties. However, after the quantitative measurements of single-molecule resistance became available, it was soon apparent that the assumption of negligible influence of the headgroup-electrode contact on the molecular resistance was oversimplified. Due to the measurement scheme of the metal--molecule-metal configuration, the contact resistance is always involved in the reported values.

Using experimental data of Escherichia coli to develop a QSAR model for predicting the photo-induced cytotoxicity of metal oxide nanoparticles.

A quantitative structure-activity relationship (QSAR) study of seventeen metal oxide nanoparticles (MNPs), in regard to their photo-induced toxicity to bacteria Escherichia coli, was developed by using quantum chemical methods. A simple and statistically significant QSAR model (F=33.83, R(2)=0.

On the size evolution of monolayer-protected gold clusters during ligand place-exchange reactions: the effect of solvents.

Ligand place-exchange (LPE) reactions are extensively applied for the post-functionalization of monolayer-protected gold clusters (MPCs) by using excessive incoming ligands to displace initial ones. However, the modified MPCs are often enlarged or degraded; this results in ill-defined size-dependent properties. The growth of MPCs essentially involves an unprotected surface that is subsequently has gold atoms added or is fused with other gold cores owing to collision.

[Biological effects of upregulated expression of transfected FOLR1 gene on SKOV3 cell lines acted by cisplatin].

Objective: To explore biological effects of up-regulated expression of transfected FOLR1 gene on SKOV3 cell lines following action by cisplatin (DDP) .

Methods: Three groups of cells originated from the same SKOV3 cell line were used in this research, including the SKOV3 cell line(blank control), the cell line transfected with lentiviral pWPI plasmid(no-load control) and the cell line transfected with FOLR1 gene via lentiviral pWPI plasmid(experimental group). Next, the mRNA and protein expression of FOLR1 gene in the three groups were detected by reverse transcription (RT) -PCR and western blot, respectively.

Quality of life of elderly Chinese rectal cancer patients after preventative anal surgery: a cohort study.

Background/aim: To assess the QOL in rectal cancer patients after preventative anal operation and to discuss the influence of age on perceived quality of life.

Methodology: A prospective study of 342 patients with rectal cancer from May 2011 to January 2012 in the gastrointestinal surgery department was randomly selected and divided into the elderly group and the young group, and the differences in their QOL assessed by the questionnaire QLQ-C30, after preventative anal surgery (7 days) were compared.

Results: A total of 207 patients met the study criteria and were divided into the elderly group (≥60 years, 107 cases) and the young group (<60 years, 100 cases).

[Analysis of cardiopulmonary bypass diluted autologous blood transfusion: a report of 5000 cases].

Objective: To explore the clinical application and experience of cardiopulmonary bypass (CPB) diluted autologous transfusion so as to provide clinical rationales for blood conservation.

Methods: At Department of Cardiothoracic Surgery, First Affiliated Hospital, Zhengzhou University, the clinical data were reviewed for 5000 CPB surgical patients without banked blood from September 1996 to March 2012 to analyze the clinical application of autologous blood transfusion and summarize the experience of depth hemodilution. No significant changes occurred in blood pressure, central venous pressure or heart rate after blood collection.

Primary mediastinal sarcoma: surgical outcomes of 21 cases.

Objectives: Primary sarcomas of the mediastinum are relatively rare. This article reviews the surgical outcomes of 21 cases diagnosed with localized mediastinal sarcomas receiving multidisciplinary treatment modalities in Sichuan province, China, from January 1996 to January 2011.

Methods: Twenty-one cases of histologically diagnosed primary mediastinal sarcoma undergoing surgical treatment were reviewed retrospectively.

Preparation and characterization of monomethoxy poly(ethylene glycol)-poly(ε-caprolactone) micelles for the solubilization and in vivo delivery of luteolin.

Luteolin (Lu) is one of the flavonoids with anticancer activity, but its poor water solubility limits its use clinically. In this work, we used monomethoxy poly(ethylene glycol)-poly(e-caprolactone) (MPEG-PCL) micelles to encapsulate Lu by a self-assembly method, creating a water-soluble Lu/MPEG-PCL micelle. These micelles had a mean particle size of 38.

Thelohanellus testudineus n. sp. (Myxosporea: Bivalvulida) infecting the skin of allogynogenetic gibel carp Carassius auratus gibelio (Bloch) in China.

A Thelohanellus species was encountered during a survey on Thelohanellus diversity of Carassius auratus gibelio (Bloch) in China. The infection is characterized by the presence of large cysts of 1.4-3.

Binding of O2 and NO to heme in heme-nitric oxide/oxygen-binding (H-NOX) proteins. A theoretical study.

The binding of O2 and NO to heme in heme-nitric oxide/oxygen-binding (H-NOX) proteins has been investigated with DFT as well as dispersion-corrected DFT methods. The local protein environment was accounted for by including the six nearest surrounding residues in the studied systems. Attention was also paid to the effects of the protein environment, particularly the distal Tyr140, on the proximal iron-histidine (Fe-His) binding.

Serotype-specific identification of Dengue virus by silicon nanowire array biosensor.

In this work, we demonstrated a silicon nanowire (SiNW) biosensing platform capable of simultaneously identifying different Dengue serotypes on a single sensing chip. Four peptide nucleic acids (PNAs), specific to each Dengue serotypes (DENV-1 to DENV-4), were spotted on different areas of the SiNW array surface, and the covalently immobilized PNA probes were then interacted with different Dengue serotypes target to establish the specificity of detection. Detection scheme is based on the changes in resistances due to accumulation of negative charges contributed by the hybridized DNA target.

Enhancement of the synthesis of n-3 PUFAs in fat-1 transgenic mice inhibits mTORC1 signalling and delays surgically induced osteoarthritis in comparison with wild-type mice.

Background: An exogenous supplement of n-3 polyunsaturated fatty acids (PUFAs) has been reported to prevent osteoarthritis (OA) through undefined mechanisms.

Objective: This study investigated the effect of alterations in the composition of endogenous PUFAs on OA, and associations of PUFAs with mammalian target of rapamycin complex 1 (mTORC1) signalling, a critical autophagy pathway in fat-1 transgenic (TG) mice.

Methods: fat-1 TG and wild-type mice were used to create an OA model by resecting the medial meniscus.

Endogenous n-3 polyunsaturated fatty acids (PUFAs) mitigate ovariectomy-induced bone loss by attenuating bone marrow adipogenesis in FAT1 transgenic mice.

Aim: To investigate the effect of endogenous n-3 polyunsaturated fatty acids (PUFAs) on bone marrow adipogenesis under osteoporosis conditions.

Methods: A mouse osteoporosis model overexpressing the FAT1 gene from Caenorhabditis elegans and converting n-6 PUFAs to n-3 PUFAs endogenously was used.

Results: The mice presented significantly lower bone marrow adiposity (adipocyte volume/tissue volume, mean adipocyte number) but increased the bone parameters (bone mineral density, bone mineral content, bone volume/total volume) in the distal femoral metaphysis.

C1GALT1 enhances proliferation of hepatocellular carcinoma cells via modulating MET glycosylation and dimerization.

Altered glycosylation is a hallmark of cancer. The core 1 β1,3-galactosyltransferase (C1GALT1) controls the formation of mucin-type O-glycans, far overlooked and underestimated in cancer. Here, we report that C1GALT1 mRNA and protein are frequently overexpressed in hepatocellular carcinoma tumors compared with nontumor liver tissues, where it correlates with advanced tumor stage, metastasis, and poor survival.

Size fraction effect on phthalate esters accumulation, bioaccessibility and in vitro cytotoxicity of indoor/outdoor dust, and risk assessment of human exposure.

Indoor and outdoor dusts from two urban centers in the Pearl River Delta, China, were analyzed and phthalate esters varied from 4.95 to 2,220 μg g(-1) in indoor dust, significantly higher than outdoor dust (1.70-869 μg g(-1)).

[Expression and clinicopathological significance of serine-257/threonine-261 phosphorylated MKK4 in colorectal carcinoma].

Objective: As an activator of JNK and p38, phosphorylated MKK4 is considered to be associated with tumor progression and prognosis. This study was to examine the expression of pMKK4 and evaluate its prognostic significance in colorectal carcinoma.

Methods: A total of 343 cases of colorectal cancer were followed up to analyze the associations between the expression of pMKK4 and various clinicopathological factors.

Exposure assessment and distribution of polychlorinated biphenyls (PCBs) contained in indoor and outdoor dusts and the impacts of particle size and bioaccessibility.

The size fraction, bioaccessibility and associated human daily intake of PCBs via indoor and outdoor dust collected from two most populated urban centers of Pearl River Delta (PRD), China, were studied. The ΣPCBs levels (ng g(-1)) in indoor (51.9-264) and outdoor (4.

Coarse-grain simulations of active molecular machines in lipid bilayers.

A coarse-grain method for simulations of the dynamics of active protein inclusions in lipid bilayers is described. It combines the previously proposed hybrid simulations of bilayers [M.-J.

Associations of CYP2E1 rs2031920 and rs3813867 polymorphisms with colorectal cancer risk: a systemic review and meta-analysis.

Cytochrome P450 2E1 (CYP2E1) is a natural enzyme involved in the metabolic activation of many carcinogens, and the functional polymorphisms in the CYP2E1 gene might have impacts on colorectal cancer risk. Many studies were published to assess the associations of CYP2E1 rs2031920 and rs3813867 polymorphisms with colorectal cancer risk, but no consistent findings were reported. A systemic review and meta-analysis of eligible studies was performed to comprehensively assess the associations above.