Sea Anemone Kunitz Peptide HCIQ2c1: Structure, Modulation of TRPA1 Channel, and Suppression of Nociceptive Reaction In Vivo.

TRPA1 is a homotetrameric non-selective calcium-permeable channel. It contributes to chemical and temperature sensitivity, acute pain sensation, and development of inflammation. HCIQ2c1 is a peptide from the sea anemone that inhibits serine proteases.

GWAS and polygenic risk score of severe COVID-19 in Eastern Europe.

Background: COVID-19 disease has infected more than 772 million people, leading to 7 million deaths. Although the severe course of COVID-19 can be prevented using appropriate treatments, effective interventions require a thorough research of the genetic factors involved in its pathogenesis.

Methods: We conducted a genome-wide association study (GWAS) on 7,124 individuals (comprising 6,400 controls who had mild to moderate COVID-19 and 724 cases with severe COVID-19).

[Prolonged spinal and sacral neurostimulation in children with pelvic organ dysfunction: preliminary analysis].

Objective: To evaluate the clinical efficacy of long-term spinal and sacral programmable neurostimulation for pelvic organ dysfunction in patients with myelodysplasia and chronic dysfunction of the bladder and rectum.

Material And Methods: A retrospective study included 32 children aged 1-17 years (mean 10.7) with myelodysplasia, pelvic organ dysfunction and ineffective therapy including botulinum therapy and exclusion of tethered spinal cord syndrome.

Dimerization of the β-Hairpin Membrane-Active Cationic Antimicrobial Peptide Capitellacin from Marine Polychaeta: An NMR Structural and Thermodynamic Study.

Capitellacin is the β-hairpin membrane-active cationic antimicrobial peptide from the marine polychaeta . Capitellacin exhibits antibacterial activity, including against drug-resistant strains. To gain insight into the mechanism of capitellacin action, we investigated the structure of the peptide in the membrane-mimicking environment of dodecylphosphocholine (DPC) micelles using high-resolution NMR spectroscopy.

Recombinant Production, NMR Solution Structure, and Membrane Interaction of the Phα1β Toxin, a TRPA1 Modulator from the Brazilian Armed Spider .

Phα1β (PnTx3-6) is a neurotoxin from the spider venom, originally identified as an antagonist of two ion channels involved in nociception: N-type voltage-gated calcium channel (Ca2.2) and TRPA1. In animal models, Phα1β administration reduces both acute and chronic pain.

Dodecapeptide Cathelicidins of Cetartiodactyla: Structure, Mechanism of Antimicrobial Action, and Synergistic Interaction With Other Cathelicidins.

In this study, dodecapeptide cathelicidins were shown to be widespread antimicrobial peptides among the clade. In particular, we investigated the dodecapeptide from the domestic goat , designated as ChDode and its unique ortholog from the sperm whale (PcDode). ChDode contains two cysteine residues, while PcDode consists of two dodecapeptide building blocks and contains four cysteine residues.

[FEATURES OF FLUID THERAPY IN CHILDREN WITH SEVERE MAJOR TRAUMA].

Background: Fluid and transfusion therapy is proved to be a required component of treating children with severe major trauma significantly influencing the case outcome.

Objective: To analyze efficiency of fluid and transfusion therapy in children with severe major trauma and assess its correspondence with current recommendations.

Materials And Methods: 150 children aged from 0 to 18 years getting treatment in intensive care units of children's city hospitals of Saint Petersburg, Archangelsk, Ufa, Samara, and Leningrad region were included in the research.

[ANALYSIS OF THE POSTOPERATIVE COURSE OF COMPLEX CONGENITAL HEART DISEASES SIMULTANEOUS CORRECTION WITH AN OPEN STERNOTOMY IN CHILDREN DURING THE FIRST MONTHS OF LIFE].

Unlabelled: In children during the first months of life delayed sternum closure is one of the few techniques increasing cardiac output after simultaneous correction of complex congenital heart defects (CHD). The aim of study was evaluating mortality, predictors of adverse outcome and frequency noncardial complications at of delayed sternum closure after correction of CHD.

Methods: Study design: a prospective, uncontrolled, cohort.

[PREVENTION OF VENTILATOR ASSOCIATED INFECTION IN NEONATES WITH RESPIRATORY DISTRESS SYNDROME].

Unlabelled: The aim of the research was to reduce the risk ventilator-associated infections (VAI) in neonates with respiratory distress syndrome.

Design: retrospective, observational, single center, historical control.

Materials And Methods: 113 newborns were included in the study.

[Anaesthetic maintenance with laryngeal mask for a laparoscopic surgery in pediatric patients].

Unlabelled: Research objective was to make a comparative assessment of haemodynamics, ventilation and oxygen status and perioperative complications during laparoscopic surgeries anaesthetic maintenance with intubation and laryngeal mask use in Pediatric patients.

Methods: A retrospective observational controlled study. STUDY TERMS: from 2000 to 2012.

[Diagnostics and treatment of sepsis in the acute phase of severe heat injury].

A prospective non-randomized, single-centered controlled study aimed the optimization of treatment and diagnostic algorithm of the early burn sepsis. 47 patients were included. The combination of bacterial wound colonization and procalcitonin blood level > or =2 ng\ml was the reliable marker of the early burn sepsis and indicates the necessity of de-escalation antibiotic therapy.

[Can the local energy minimization refine the PDB structures of different resolution universally?].

The local energy minimization was statistically validated as the refinement strategy for PDB structure pairs of different resolution. Thirteen pairs of structures with the only difference in resolution were extracted from PDB, and the structures of 11 identical proteins obtained by different X-ray diffraction techniques were represented. The distribution of RMSD value was calculated for these pairs before and after the local energy minimization of each structure.

[Genetic markers of predisposition to infectious complications in neonatal infants with respiratory distress syndrome].

Neonatal respiratory distress syndrome (NRDS) is the commonest cause of death and morbidity of the newborn. A genetic risk for NRDS is currently recognized. The aim of this study was to determine whether there was an association of the polymorphisms of the SP-B, SP-D genes and the interleukins (IL)-1alpha, IL-10 genes with the development of infectious complications in neonatal infants with the respiratory distress syndrome.

[Estimation of the validity of pediatric severity rating scales in multidisciplinary children's intensive care units].

The aim of this study was to estimate the validity of pediatric severity rating scales at general pediatric intensive care units. This was a prospective, observational multicenter study that was performed from November 1, 2007, to January 31, 2008. The end points were the estimation of the severity of disease, by using the PRISM, PIM II, RELOD scores, and 28-day survival.

[High epidural anesthesia as a component of anesthetic support for cardiosurgical interventions in infants].

The aim of the study was to determine whether it was expedient to include epidural anesthesia into a complex of anesthetic support of surgical correction of ventricular septal defect in infants. This was a prospective, nonrandomized, monocenter case-control study. Seventy-nine infants met the inclusion criteria.

[Elective video-laparoscopic sanation for the treatment of general peritonitis at children].

Results of traditional and video-laparoscopic relaparotomy at children with appendicular general peritonitis are analyzed. Design--one-center, retrospective, case-control study. It is demonstrated that variant of relaparotomy does not correlate with survival and intensive care content.

[Comparative estimation of currently available approaches to early identification of severe acute pancreatitis].

It is difficult to precisely predict the severity of each specific patient with acute pancreatitis (AP), by using conventional statistical methods or single clinical and laboratory criteria. By using the patient database, the authors developed an artificial neuronal network (ANN) to predict the severity of AP and compared it with the Ranson, Imrie/Glasgow, APACHE II, and Physiological Condition Severity scoring systems, ultrasound/radiological criteria, and linear regression analysis. ANN was found to be significantly better than all the scoring systems, but not better than a linear regression model (the area under the receiver operating characteristic curves were equal to 0.

[Physicochemical properties of multicomponent polyhydroxyalkanoates].

X-ray structure analysis, IR spectrometry, differential thermal analysis, and viscosimetry have been used to investigate the properties of novel five-component polyhydroxyalkanoates formed by short- and medium-chain-length monomers synthesized by the bacterium Wautersia eutropha B5786. As the molar fraction of hydroxyhexanoate contained in polyhydroxyalkanoates samples increased from 2.5 to 18.

[Prognostic value of the pattern of multiple organ dysfunctions in severe acute pancreatitis].

A relationship was studied between the outcomes of severe acute pancreatitis and the pattern and multiple organ dysfunctions in 72 patients. Organ dysfunction was observed in 39 (54.2%) patients: multiple organ dysfunction (MOD) in 20 (27.

[The effect of organ dysfunction on the outcomes of acute destructive pancreatitis].

The relationships between outcomes of severe acute pancreatitis (SAP) and organ dysfunction in 72 patients were studied. A total of 39 (54.2%) patients had organ dysfunction, 20 (27.

[Diagnosis and treatment of ventilator-associated pneumonia in children].

The purpose of the investigation was to optimize the diagnosis and treatment of ventilator-associated pneumonia (VAP) in children. The prevalence, etiology, and onset of VAP in a pediatric intensive care unit, and the diagnostic value the clinical pulmonary infection score scale were studied. The prevalence of VAP was found to be 37.

[Modern approaches to volemic compensation in children with severe thermal injury during acute period].

To determine the minimally allowable levels of oxygen delivery and consumption in infants with acute severe thermal injury, the authors studied the oxygen transport system in 36 patients aged 1 year and 2 months to 3 years who had burns of more than 15%, but less than 50% of the body surface in February 2001 to January 2004. The findings indicate that in infants, the acute period of thermal injury is characterized by oxygen debts despite the hyperdynamic circulatory regimen. By calculating the coefficients of paired correlation and deriving the linear regression of oxygen uptake with other parameters of the oxygen transport system, the authors estimated the minimum allowable levels of performance of the oxygen transport system: less than 623.

[Comparative assessment of rocuronium bromide and cysatracurium besylate used during endosurgical operations in children].

The authors have studied the effects of the muscular relaxants rocuronium bromide and cysatracurium besylate on neuromuscular conduction, respiration mechanics, and hemodynamics in 120 children during endosurgical operations. The paper comparatively assesses the muscular relaxants and the procedures of their use, which made it possible to ensure controlled and deep relaxation.