Organelle Interactions in Plant Cells.

The sequestration of enzymes and associated processes into sub-cellular domains, called organelles, is considered a defining feature of eukaryotic cells. However, what leads to specific outcomes and allows a eukaryotic cell to function singularly is the interactivity and exchanges between discrete organelles. Our ability to observe and assess sub-cellular interactions in living plant cells has expanded greatly following the creation of fluorescent fusion proteins targeted to different organelles.

Proximity driven plastid-nucleus relationships are facilitated by tandem plastid-ER dynamics.

Peri-nuclear clustering (PNC) of chloroplasts has largely been described in senescent and pathogen- or reactive oxygen species-stressed cells. Stromules, tubular plastid extensions, are also observed under similar conditions. Coincident observations of PNC and stromules associate the two phenomena in facilitating retrograde signaling between chloroplasts and the nucleus.

Effects of Immersion on Knowledge Gain and Cognitive Load in Additive Manufacturing Process Education.

Although the additive manufacturing (AM) market continues to grow, industries face barriers to AM adoption due to a shortage of skilled designers in the workforce that can apply AM effectively to meet this demand. This shortage is attributed to the high cost and infrastructural requirements of introducing high- barrier-to-entry AM processes such as powder bed fusion (PBF) into in-person learning environments. To meet the demands for a skilled AM workforce, it is important to explore other mediums of AM education, such as computer-aided instruction (CAI) and virtual reality (VR), which can increase access to hands-on learning experiences for inaccessible AM processes.

Using ER-Targeted Photoconvertible Fluorescent Proteins in Living Plant Cells.

Photoconvertible fluorescent proteins (pcFPs) enable differential coloring of a single organelle. Several pcFP-based probes have been targeted to the endoplasmic reticulum (ER) and can serve as useful tools to study ER dynamics and interactions with other organelles. Here, we describe the procedure to conduct live-cell imaging experiments using ER-targeted pcFP-based probes.

Synergistic effect of pyrene and heavy metals (Zn, Pb, and Cd) on phytoremediation potential of Medicago sativa L. (alfalfa) in multi-contaminated soil.

The environment in India is contaminated with polycyclic aromatic hydrocarbons (PAHs) due to the occurrence of large anthropogenic activities, i.e., fuel combustion, mineral roasting, and biomass burning.

Membrane contacts with the endoplasmic reticulum modulate plastid morphology and behaviour.

Plastid behaviour often occurs in tandem with endoplasmic reticulum (ER) dynamics. In order to understand the underlying basis for such linked behaviour we have used time-lapse imaging-based analysis of plastid movement and pleomorphy, including the extension and retraction of stromules. Stable transgenic plants that simultaneously express fluorescent fusion proteins targeted to the plastid stroma, and the ER along with BnCLIP1-eGFP, an independent plastid envelope localized membrane contact site (MCS) marker were utilized.

Functional neurological disorder: A common reason for a neurology inpatient referral.

Background And Purpose: In 2021, the European Academy of Neurology's training requirements were updated to include functional neurological disorder (FND) as a core topic for the first time. To reinforce these changes, we aimed to understand the proportion of inpatients (in non-neurology settings) who are diagnosed with FND.

Methods: We prospectively collected data on diagnoses made after inpatient ward reviews from neurology trainees at three tertiary neurology centres in Scotland from April to September 2021.

Microbial-assisted phytodegradation for the amelioration of pyrene-contaminated soil using and with alfalfa and sunflower.

Environmental pollution caused by polycyclic aromatic hydrocarbons (PAHs) jeopardizes nature. PAHs are the most toxic, mutagenic, and carcinogenic pollutants and their cleanup is important for the environment. In the current research, to assess and evaluate three remediation strategies for pyrene removal from the soil, a pot experiment was performed: (a) bioremediation with  and , (b) phytoremediation with sunflower () and alfalfa ( L.

Matrix obstructions cause multiscale disruption in collective epithelial migration by suppressing leader cell function.

During disease and development, physical changes in extracellular matrix cause jamming, unjamming, and scattering in epithelial migration. However, whether disruptions in matrix topology alter collective cell migration speed and cell-cell coordination remains unclear. We microfabricated substrates with stumps of defined geometry, density, and orientation, which create obstructions for migrating epithelial cells.

Reciprocal intra- and extra-cellular polarity enables deep mechanosensing through layered matrices.

Adherent cells migrate on layered tissue interfaces to drive morphogenesis, wound healing, and tumor invasion. Although stiffer surfaces are known to enhance cell migration, it remains unclear whether cells sense basal stiff environments buried under softer, fibrous matrix. Using layered collagen-polyacrylamide gel systems, we unveil a migration phenotype driven by cell-matrix polarity.

Comparative analysis of remediation efficiency and ultrastructural translocalization of polycyclic aromatic hydrocarbons in , and .

Polycyclic aromatic hydrocarbons (PAHs) are semi-volatile anthropogenic contaminants that can damage soil fertility and threaten the environment due to their hazardous effects on various ecological parameters. The experimental objective was divided into two parts because PAHs are always present in mixtures. The toxicity of anthracene, phenanthrene, pyrene, and fluoranthene was examined and investigated the potential of three phytoremediator plants species viz , , and for remediation and translocation of individual PAH.

Mechanically primed cells transfer memory to fibrous matrices for invasion across environments of distinct stiffness and dimensionality.

Cells sense and migrate across mechanically dissimilar environments throughout development and disease progression. However, it remains unclear whether mechanical memory of past environments empowers cells to navigate new, three-dimensional extracellular matrices. Here, we show that cells previously primed on stiff, compared with soft, matrices generate a higher level of forces to remodel collagen fibers and promote invasion.

Isolation, purification and characterization of quercetin from peels; its antimicrobial, antioxidant and cytotoxicity evaluations.

The peels of C.s are produced in large quantities in food processing industries and as kitchen-waste, resulting into tremendous loss of valuable bioactive components. Considering this, the flavanoids from C.

A Cdh3-β-catenin-laminin signaling axis in a subset of breast tumor leader cells control leader cell polarization and directional collective migration.

Carcinoma dissemination can occur when heterogeneous tumor and tumor-stromal cell clusters migrate together via collective migration. Cells at the front lead and direct collective migration, yet how these leader cells form and direct migration are not fully appreciated. From live videos of primary mouse and human breast tumor organoids in a 3D microfluidic system mimicking native breast tumor microenvironment, we developed 3D computational models, which hypothesize that leader cells need to generate high protrusive forces and overcome extracellular matrix (ECM) resistance at the leading edge.

Value added bioactive compounds from fruits & vegetables waste for assessing their antimicrobial activity.

Since ancient time, plants and there parts have been used widely against dreadful pathogens due to ability of killing microbes. Waste from fruits and vegetables are pulling in more interest in exploration due to their therapeutic properties such as anti-pathogenic activity. In the present study antimicrobial and cytotoxicity properties of herbal combination prepared from peels of Allium cepa, Cucumis sativus, Citrus reticulata, and Mangifera indica were investigated.

Phytoaccumulation of zinc from contaminated soil using ornamental plants species L. and L.

Intensive research on hyperaccumulator plant species provides an alternative method to cleanup heavy metal contaminated sites using these plants. and are suitable hyperaccumulator plant species for removing zinc (Zn) from contaminated soil because of their high phytoremediation effectiveness. The present study focused on to evaluate comparative efficacy of Zn accumulation using and .

Tandem Protocol for the Synthesis of Pyrano[3,2-]quinolone Derivatives Using Taurine as a Green Bio-Organic Catalyst in Aqueous Medium.

A series of pyrano[3,2-]quinolone derivatives has been synthesized in the presence of taurine (2-aminoethanesulfonic acid) as a green bio-organic catalyst and water as the solvent. The target compounds were synthesized through the three-component reaction between aldehydes, malononitrile/ethylcyanoacetate, and 4-hydroxy-1-methyl-2(1)-quinolone. The advantages of this protocol are excellent yields of products, short reaction times, cost efficiency, atom economy, and a simple work-up procedure with no need for extra purification techniques.

Surgical Management and Prosthetic Rehabilitation of Dentigerous Cyst Associated with Impacted Mandibular Canine: A Case Report.

The most frequent odontogenic developing cyst linked with an impacted tooth is the dentigerous cyst. They are normally asymptomatic and are identified during routine radiography tests to see if a tooth is missing from the arch. This case report is designed to demonstrate how an enucleated dentigerous cyst with impacted teeth led to the spontaneous recovery of a bone defect without the need for bone graft.

Comparative evaluation of cadmium phytoremediation potential of five varieties of L.

is a potential metal accumulator plant, which can find application in cadmium (Cd) phytoremediation and provide economic gains in terms of oil yield. This study is focused on Cd accumulation analysis, physiological and biochemical responses of five varieties of (DRSF-108, DRSF-113, LSFH-171, Phule Bhaskar and KBSH-44). Plantlets of all varieties were treated with various Cd concentrations (10, 50, 100, 300 and 500 mg kg) for 20, 40 and 60 days.

Silica nanoparticles as novel sustainable approach for plant growth and crop protection.

Agriculture crops encounter several biotic and abiotic stresses, including pests, diseases, nutritional deficits, and climate change, which necessitate the development of new agricultural technologies. By developing nano-based fertilizers, insecticides and herbicides, and early disease diagnostics, nanotechnology may help to increase agricultural crop quality and production. The application of silica nanoparticles (SiNPs) may be the solution for increasing the yield to combat the agriculture crisis in the near future.

Opportunistic Non-Governmental Organisation Delivery of a Virtual Stop Smoking Service in England during the COVID-19 Lockdown.

Smoking cessation services have rapidly transformed during the COVID-19 pandemic. Changes include pivoting from face-to-face to telephone and video call support, remote provision of stop smoking aids and more flexible appointments. This study reports an evaluation of a charity-led smoking cessation service rapidly conceived and launched in this context.

The ER Is a Common Mediator for the Behavior and Interactions of Other Organelles.

Optimal functioning of a plant cell depends upon the efficient exchange of genetic information, ions, proteins and metabolites between the different organelles. Intuitively, increased proximity between organelles would be expected to play an important role in facilitating exchanges between them. However, it remains to be seen whether under normal, relatively non-stressed conditions organelles maintain close proximity at all.

Application of agro-waste-mediated silica nanoparticles to sustainable agriculture.

Use of green agronomic techniques for plant development and crop protection is essential for environmental sustainability. The current research investigates a more efficient and long-term technique of manufacturing silica nanoparticles (SiO NPs) from agricultural waste (sugarcane bagasse and corn cob). SiO NPs were synthesized by calcinations of waste residues in muffle furnace with varying temperatures (400-1000 °C)/2 h in the present of static air.