UAV-assisted real-time evidence detection in outdoor crime scene investigations.

Nowadays, a plethora of unmanned aerial vehicles (UAVs) designs that significantly vary in size, shape, operating flight altitude, and flight range have been developed to provide multidimensional capabilities across a wide range of military and civil applications. In the field of forensic and police applications, drones are becoming increasingly used instead of helicopters to assist field officers to search for vulnerable missing persons or to target criminals in crime hotspots, and also to provide high-quality data for the documentation and reconstruction of the forensic scene or to facilitate evidence detection. This paper aims to examine the contribution of UAVs in real-time evidence detection in outdoor crime scene investigations.

Analysis of a crucial interaction between the coronavirus nucleocapsid protein and the major membrane-bound subunit of the viral replicase-transcriptase complex.

The coronavirus nucleocapsid (N) protein comprises two RNA-binding domains connected by a central spacer, which contains a serine- and arginine-rich (SR) region. The SR region engages the largest subunit of the viral replicase-transcriptase, nonstructural protein 3 (nsp3), in an interaction that is essential for efficient initiation of infection by genomic RNA. We carried out an extensive genetic analysis of the SR region of the N protein of mouse hepatitis virus in order to more precisely define its role in RNA synthesis.

Extended Goal Recognition: Lessons from Magic.

The "science of magic" has lately emerged as a new field of study, providing valuable insights into the nature of human perception and cognition. While most of us think of magic as being all about deception and perceptual "tricks", the craft-as documented by psychologists and professional magicians-provides a rare practical demonstration and understanding of goal recognition. For the purposes of human-aware planning, goal recognition involves predicting what a human observer is most likely to understand from a sequence of actions.

Application of viewshed analysis and probability mapping for search area determination based on the Moors murders on Saddleworth Moor, the United Kingdom.

Geographical Information System (GIS) is underutilized within the forensic discipline, especially concerning the discovery of clandestine graves. GIS, viewshed analysis, and probability maps are being introduced into desk-based research both for archaeology and forensic purposes. This study aims to investigate how useful GIS can be to narrow down large search areas when locating clandestine graves.

Pain Management Stewardship Programs, Part I: A Review of Legislative and Regulatory Changes.

Opioid use is classified as an epidemic by many due to the impact of these medications on society. Federal and state laws for prescribing and dispensing opioids have changed rapidly in a short period of time to hopefully balance proper pain control with their use. Pharmacy directors must be informed of these rapid changes to effectively work as part of any opioid stewardship team.

A common partitivirus infection in United States and Czech Republic isolates of bat white-nose syndrome fungal pathogen Pseudogymnoascus destructans.

The psychrophilic (cold-loving) fungus Pseudogymnoascus destructans was discovered more than a decade ago to be the pathogen responsible for white-nose syndrome, an emerging disease of North American bats causing unprecedented population declines. The same species of fungus is found in Europe but without associated mortality in bats. We found P.

Coronavirus genomic RNA packaging.

RNA viruses carry out selective packaging of their genomes in a variety of ways, many involving a genomic packaging signal. The first coronavirus packaging signal was discovered nearly thirty years ago, but how it functions remains incompletely understood. This review addresses the current state of knowledge of coronavirus genome packaging, which has mainly been studied in two prototype species, mouse hepatitis virus and transmissible gastroenteritis virus.

Inclusion of Real-Time Hand Hygiene Observation and Feedback in a Multimodal Hand Hygiene Improvement Strategy in Low-Resource Settings.

Importance: Hand hygiene adherence monitoring and feedback can reduce health care-acquired infections in hospitals. Few low-cost hand hygiene adherence monitoring tools exist in low-resource settings.

Objective: To pilot an open-source application for mobile devices and an interactive analytical dashboard for the collection and visualization of health care workers' hand hygiene adherence data.

The application of low-altitude near-infrared aerial photography for detecting clandestine burials using a UAV and low-cost unmodified digital camera.

Aerial photography and remote sensing has been carried out in the past by numerous different platforms, utilizing imaging from across the electromagnetic (EM) spectrum to gain information about the earth. These techniques have additionally been found effective when locating mass graves and single clandestine graves created by perpetrators when concealing homicide victims. Applications for performing aerial photography and remote sensing are costly and therefore usually overlooked by police investigators, resulting in employing more contemporary geophysical methods for locating burials.

Health issues of refugees attending an infectious disease refugee health clinic in a regional Australian hospital.

Background And Objectives: Refugees in Australia present with conditions different to those of the general population. The aim of this study was to review the reasons for referral, prevalence of conditions and treatment outcomes for refugee patients attending a specialist referral clinic in regional Victoria.

Method: A retrospective review was undertaken of patients attending the refugee health clinic at University Hospital Geelong from January 2007 to December 2012.

Regulation of Camphor Metabolism: Induction and Repression of Relevant Monooxygenases in Pseudomonas putida NCIMB 10007.

For the first time, the differential rates of synthesis of all the key monooxygenases involved in the catabolism by NCIMB 10007 of bicyclic ()-camphor to ∆-3,4,4-trimethylpimelyl-CoA, the first aliphatic pathway intermediate, have been determined to help establish the relevant induction profile of each of the oxygen-dependent enzymes. The efficacy of both relevant substrates and pathway metabolites as inducers has been established. Further, inhibitors with characterised functionality have been used to indicate that the pertinent regulatory controls operate at the level of transcription of the corresponding genes.

Optimization of the blood culture pathway: a template for improved sepsis management and diagnostic antimicrobial stewardship.

Laboratory processing of blood cultures has remained static over the past 30 years, despite increasing antibiotic resistance and advances in analyser design. At the study hospital, siting the blood culture analyser in the blood sciences laboratory and optimizing the pre-analytical and analytic phases of blood culture management resulted in a reduction in the time taken to detect most blood culture isolates to <12h. Fifty percent of positive blood cultures containing Escherichia coli were definitively reported with antibiotic susceptibilities in <24h.

Alignment of Epidemiology Practice and Academic Competencies through Effective Collaboration.

Background: Online learning has recently garnered increased attention as technology use in the classroom grows. However, most of the published approaches regarding this topic in postgraduate education centers on clinical environments. Models of partnerships between applied public health agencies and academic centers to produce mutually beneficial online learning opportunities for graduate-level public health courses have not been explored in the literature.

A key role for the carboxy-terminal tail of the murine coronavirus nucleocapsid protein in coordination of genome packaging.

The prototype coronavirus mouse hepatitis virus (MHV) exhibits highly selective packaging of its genomic positive-stranded RNA into assembled virions, despite the presence in infected cells of a large excess of subgenomic viral mRNAs. One component of this selectivity is the MHV packaging signal (PS), an RNA structure found only in genomic RNA and not in subgenomic RNAs. It was previously shown that a major determinant of PS recognition is the second of the two RNA-binding domains of the viral nucleocapsid (N) protein.

Analyses of Coronavirus Assembly Interactions with Interspecies Membrane and Nucleocapsid Protein Chimeras.

Unlabelled: The coronavirus membrane (M) protein is the central actor in virion morphogenesis. M organizes the components of the viral membrane, and interactions of M with itself and with the nucleocapsid (N) protein drive virus assembly and budding. In order to further define M-M and M-N interactions, we constructed mutants of the model coronavirus mouse hepatitis virus (MHV) in which all or part of the M protein was replaced by its phylogenetically divergent counterpart from severe acute respiratory syndrome coronavirus (SARS-CoV).

Dissection of amino-terminal functional domains of murine coronavirus nonstructural protein 3.

Unlabelled: Coronaviruses, the largest RNA viruses, have a complex program of RNA synthesis that entails genome replication and transcription of subgenomic mRNAs. RNA synthesis by the prototype coronavirus mouse hepatitis virus (MHV) is carried out by a replicase-transcriptase composed of 16 nonstructural protein (nsp) subunits. Among these, nsp3 is the largest and the first to be inserted into the endoplasmic reticulum.

Development of a high-value care subscore on the internal medicine in-training examination.

Background: Although high-value care (HVC) that balances benefits of tests or treatments against potential harms and costs has been a recently emphasized competency for internal medicine (IM) residents, few tools to assess residents' knowledge of HVC are available.

Objective: To describe the development and initial results of an HVC subscore of the Internal Medicine In-Training Examination (IM-ITE).

Design: The HVC concepts were introduced to IM-ITE authors during question development.

In the clinic. Insomnia.

This issue provides a clinical overview of Insomnia focusing on prevention, diagnosis, treatment, practice improvement, and patient information. The content of In the Clinic is drawn from the clinical information and education resources of the American College of Physicians (ACP), including ACP Smart Medicine and MKSAP (Medical Knowledge and Self-Assessment Program). Annals of Internal Medicine editors develop In the Clinic from these primary sources in collaboration with the ACP's Medical Education and Publishing divisions and with the assistance of science writers and physician writers.

Multiple vitamin deficiencies in a patient with a history of chronic alcohol excess and self-neglect in the UK.

We report a case of inadequate diet (caused by extreme self-neglect and alcohol excess) which led to chronic severe deficiencies of vitamins A, D and E. At presentation the patient had widespread follicular hyperkeratosis of the skin, keratomalacia of both eyes and a severe cognitive impairment. He responded well to treatment including high dose parenteral vitamins, but lasting impairments in his vision and cognition have caused permanent disability.

Developing an academic health department in Northeast Tennessee: a sustainable approach through student leadership.

In an effort to bridge the gap between public health practice and academia, the Health Resources and Services Administration-funded Tennessee Public Health Training Center (LIFEPATH) has supported establishment of an academic health department (AHD) involving the East Tennessee State University College of Public Health (COPH) and the Sullivan County Regional Health Department (SCRHD). The SCRHD identified a need to increase internal capacity to conduct ongoing community health assessment and community-centered practice. Similarly, the COPH recognized the need to expand evidence-based practice implementation and evaluation opportunities for public health students.

Hospitalist career decisions among internal medicine residents.

Background: Hospital medicine is a rapidly growing field of internal medicine. However, little is known about internal medicine residents' decisions to pursue careers in hospital medicine (HM).

Objective: To identify which internal medicine residents choose a career in HM, and describe changes in this career choice over the course of their residency education.

Recognition of the murine coronavirus genomic RNA packaging signal depends on the second RNA-binding domain of the nucleocapsid protein.

Unlabelled: The coronavirus nucleocapsid (N) protein forms a helical ribonucleoprotein with the viral positive-strand RNA genome and binds to the principal constituent of the virion envelope, the membrane (M) protein, to facilitate assembly and budding. Besides these structural roles, N protein associates with a component of the replicase-transcriptase complex, nonstructural protein 3, at a critical early stage of infection. N protein has also been proposed to participate in the replication and selective packaging of genomic RNA and the transcription and translation of subgenomic mRNA.

Characterization of a critical interaction between the coronavirus nucleocapsid protein and nonstructural protein 3 of the viral replicase-transcriptase complex.

The coronavirus nucleocapsid protein (N) plays an essential structural role in virions through a network of interactions with positive-strand viral genomic RNA, the envelope membrane protein (M), and other N molecules. Additionally, N protein participates in at least one stage of the complex mechanism of coronavirus RNA synthesis. We previously uncovered an unanticipated interaction between N and the largest subunit of the viral replicase-transcriptase complex, nonstructural protein 3 (nsp3).