CMV plasma DNAemia and risk of cancer among HIV-infected patients: a case-control study nested in the ANRS CO3 Aquitaine Cohort, France, 2002-2007.

Background: CMV reactivation, which enhances immune senescence, could be associated with a higher risk of cancer.

Objectives: We compared the prevalence of positive CMV DNAemia in HIV-infected patients with and without cancer.

Study Design: This case-control study, nested in the ANRS-CO3 Aquitaine Cohort, included patients with a first diagnosis of cancer (2002-2007) as cases.

Two novel plant cDNAs homologous to animal type-2 phosphatidate phosphatase are expressed in cowpea leaves and are differently regulated by water deficits.

Two cDNAs encoding putative phosphatidate phosphatases (PAPs) designated VuPAP-alpha and VuPAP-beta were cloned in cowpea (Vigna unguiculata L.) leaves. The predicted proteins have six membrane-spanning regions in common with animal type-2 PAPs.

Binding of two anthranilic acid derivatives to human albumin, erythrocytes, and lipoproteins: evidence for glafenic acid high affinity binding.

The binding of two anthranilic acid derivatives, glafenic and floctafenic acids, to human erythrocytes and plasma proteins has been investigated in vitro by equilibrium dialysis. Despite their close chemical structures it was shown that the binding of the two compounds to serum albumin, lipoproteins, and erythrocytes was dramatically different both in quality and quantity. Using various techniques including fluorometry and circular dichroism, it was shown that glafenic acid binds to the human serum albumin (HSA) warfarin/azapropazone site and that floctafenic acid binds to both warfarin/azapropazone and benzodiazepine sites.

Assessment of erythrocyte deformability by constant flow filtration technique: analysis of factors influencing the initial pressure.

When filtering red blood cells (RBC) resuspended into buffer at constant flow, the pressure-time curve is dependent on erythrocyte deformability (E.D) and also on the plugging of pores by RBC or leukocytes (WBC). To understand the influence of these 3 factors, we used an apparatus with: 1) a constant flow syringe (Bioblock) (1.

[Blood microfiltration methods].

Red cell deformability can be approached by filtration through narrow channels either at constant pressure or at constant flow. A very simplified constant pressure method is described, measuring relative flow of 8% hematocrit red cells resuspended in buffer. A constant flow method is also described, using again 8 to 10% hematocrit, which the authors consider as more physiological than the very low hematocrits used by others.

Pentoxifylline and cerebrovascular diseases.

Pentoxifylline is a drug known to increase red cell deformability, thus allowing the 7- to 8-micrometers diameter red cell to flow through the narrowest capillaries of 3-5 micrometers diameter. Red cell deformability is reduced in various forms of cerebrovascular impairment. Pentoxifylline can be effective in these states, not only in restoring red cell deformability, but also on hemodynamic and clinical parameters.

From Virchow to red cells (the unended quest).

For Virchow vessel wall, coagulability and flow were the three eventual culprits of thrombogenesis. Pharmacological progress in the three fields has not been parallel. Strangely enough they have found possible therapy in the order of their description: first the vessel wall with vasodilators.

Pharmacological improvement of altered red cell deformability.

Red cell deformability can be approached on a large clinical scale using filtration methods. Filtration and/or deformability have been shown to be reduced during vascular diseases. The biochemistry of red cell deformability can help to understand this phenomenon.

[Red cell filterability, smoking and cardiovascular risk factors (author's transl)].

Red cell deformability, which allows cells of 7 mu diameter to flow through capillaries not larger than 3 mu, can be approached by the measure of blood filterability on nuclepore 5 mu filters. Filterability is reduced in arterial diseases. We have, in 72 patients, correlated red cell filterability, with the number of cardiovascular risk factors present high blood pressure, overweight, diabetes, hyperuricemia, hyperlipemia smoking).

Red cell deformability: physiological, clinical and pharmacological aspects.

Twenty five thousand billions of red cells circulate through 300 miles of capillary network. The red cell diameter is 7 to 8 microns and the capillary diameter 3 to 5 micron. Red cells deformability is therefore an essential condition for survival.

High prekallikrein levels during pregnancy.

Prekallikrein levels are significantly increased during pregnancy. The increase is neither due to an excess of Hageman factor or of an activator, nor to the decrease of an inhibitor.