Prognostic Value of Ceramide Dynamics in Patients with Acute Coronary Syndrome.

A dynamic study of ceramide concentrations and their association with recurrent event risk could enhance our understanding of cardiovascular complications. To assess the prognostic value of ceramide concentrations (Cer(d18:1/16:0), Cer(d18:1/18:0), Cer(d18:1/24:1), Cer(d18:1/24:0)) and their dynamics in combination with standard clinical and laboratory parameters and therapeutic interventions in ACS patients. Among 110 ACS patients, triple blood sampling was performed for targeted lipidomic analysis using high-performance liquid chromatography-tandem mass spectrometry.

SecM leader peptide as an allosteric translation inhibitor: a molecular dynamics study.

The SecM leader peptide regulates translation of the SecA protein, being a part of the Sec translocase, that reversibly arrests the ribosome. In the present study the structure of the SecM complex with the E. coli A/A,P/P-ribosome was obtained by means of docking and molecular dynamics simulation methods.

Rights and responsibilities: Women leadership for health in Kyrgyzstan.

The World Health Organization (WHO) is committed to empowering countries by implementing a gender, equity, and human rights approach in the health sector. The objective of this gender and inclusion analysis is to assess potential gender disparities of health sector management in the Kyrgyz Republic. The employed mixed-method approach takes advantage of data triangulation.

High Risk of Heart Tumors after COVID-19.

Unlabelled: An emergence of evidence suggests that severe COVID-19 is associated with an increased risk of developing breast and gastrointestinal cancers. The aim of this research was to assess the risk of heart tumors development in patients who have had COVID-19.

Methods: A comparative analysis of 173 heart tumors was conducted between 2016 and 2023.

Maternal uniparental disomy of chromosome 21 as a cause of pseudo-exclusion from paternity.

Uniparental disomy (UPD) is a rare chromosomal condition, which apart from its importance in medical genetics can affect an outcome of parentage DNA testing, often causing pseudo exclusions. We describe a case of trio paternity test using 24 informative STR loci with potential exclusion at 2 systems located on chromosome 21. Consequent genotyping of an additional 25 autosomal and 27 Y-specific STRs revealed one other inconsistency, also located on this chromosome.

Morphological Changes in the Myocardium of Patients with Post-Acute Coronavirus Syndrome: A Study of Endomyocardial Biopsies.

Unlabelled: The clinical manifestation study of post-acute sequelae of SARS-CoV-2 infection (PASC) has shown a lack of knowledge regarding its morphology and pathogenesis. The aim of this research was to investigate morphological manifestations of PASC in the myocardium.

Materials And Methods: The study included 38 patients requiring endomyocardial biopsy (EMB) during the post-acute phase of coronavirus infection and a control group including patients requiring EMB prior to the SARS-CoV-2 pandemic.

Paternity pseudo-exclusion caused by tetragametic chimerism in a gestational surrogacy case.

Chimerism is a condition when an organism is composed of two or more populations of genetically distinct cells. Chimerism often produces curious results of medical and genetic investigations and could be a major cause of false negative conclusions in parentage testing. Here we describe a paternity pseudo-exclusion due to tetragametic chimerism in a gestational surrogacy case originated in a fertility clinic.

Genetic diversity and forensic parameters of autosomal STR markers included PowerPlex® CS7 panel in the general population of the Russian Federation.

PowerPlex® CS7 multiplex is commonly used as a source of supplementary markers in parentage and kinship studies. We analysed a total of 687 unrelated individuals from 94 geographically different localities across all Federal Districts of the Russian Federation and provide forensically important parameters and allele frequencies. The paper also presents results of an intra-population genetic diversity study between the populations of the Federal Districts and comparison with populations from various regions of the world.

Long-term outcomes of third-line therapy with tyrosine kinase inhibitors in chronic phase chronic myeloid leukemia: A real-life experience.

Introduction: Tyrosine kinase inhibitor (TKI) therapy has greatly improved the prognosis of patients with chronic myeloid leukemia (CML), improving the survival expectancy of patients with chronic phase (CP) CML to that of the general population. However, despite these advances, nearly 50% of patients with CP CML experience failure to respond to frontline therapy, and most fail to respond to the subsequent second-line TKI. Treatment guidelines for patients failing second-line therapy are lacking.

Electronic Structure of Pb Adsorbed Surfaces of Intrinsic Magnetic Topological Insulators.

Recently discovered intrinsic magnetic topological insulators (IMTIs) constitute a unique class of quantum materials that combine magnetism and nontrivial topology. One of the most promising applications of these materials is Majorana fermion creation; Majorana fermions are expected to arise when a superconductor is in contact with the surface of an IMTI. Here we study the adsorption of Pb ultrathin films on top of IMTIs of various stoichiometries.

Genetic diversity and forensic parameters of 12 X-STR included in Argus X-12® marker panel in the population of the Russian Federation.

Background: X-chromosome specific short tandem repeats (X-STR) are indispensable for parentage analysis, kinship determination and forensic casework.

Aim: To determine allelic frequencies and forensic parameters of the 12 X-STR markers from the Investigator Argus X-12 kit panel for the population of the Russian Federation.

Subjects And Methods: We analysed 803 unrelated individuals from 58 localities across the Russian Federation.

COVID-19, Acute Lymphoblastic Leukemia, and Down Syndrome: A Short Review and a Case Report.

SARS-CoV-2 which causes COVID-19 has been a great challenge to treat and deal with; despite strict measures that had been implemented by governments and organizations, worrying rates of morbidity and mortality are still being reported. Although available data have expressed that moderate or even mild forms of the disease are expected amongst most pediatrics cases, minimal data are available on the prognosis and the disease's complications on the immunocompromised, especially oncology patients. We report a case of relapsed precursor B-cell acute lymphoblastic leukemia of a child with Down syndrome and COVID-19 and outline the treatment regimen that we used.

Population genetic data and forensic parameters of the 27 Y-STR panel Yfiler Plus in Russian population.

This is the first report of a nation-wide study of the 27-STR Yfiler® Plus panel in the population of the Russian Federation. A total of 691 unrelated males from 89 locations split into seven subpopulations were analysed. Forensic parameters, haplotype and allele frequencies and presented.

Investigation of Allosteric Effect of 2,8-Dimethylation of A2503 in E. coli 23S rRNA by Molecular-Dynamics Simulations.

Ribosome is a molecular machine that synthesizes all cellular proteins. It also is a target of about half of the clinically used antibiotics. Adaptive chemical modification of ribosomal RNAs residues is one of the ways to provide resistance to certain antibiotics.

Fungal literature records database of the Northern West Siberia (Russia).

Background: Mycological research in the Northern part of West Siberia has now become sufficient for review and digitisation as over 460 scientific works have been completed mainly since the beginning of the 20th century. The history of research in the region started from isolated studies at the beginning of the 20th century, but regular and systematic research started from the 1970s. Over the following decades, several dozens of researchers have worked in the area, but the reported occurrences were scattered amongst a broad variety of publications, mainly hardly available.

A noncanonical binding site of linezolid revealed via molecular dynamics simulations.

Linezolid, an antibiotic of oxazolidinone family, is a translation inhibitor. The mechanism of its action that consists in preventing the binding of aminoacyl-tRNA to the A-site of the large subunit of a ribosome was embraced on the basis of the X-ray structural analysis of the linezolid complexes with vacant bacterial ribosomes. However, the known structures of the linezolid complexes with bacterial ribosomes poorly explain the linezolid selectivity in suppression of protein biosynthesis, depending on the amino acid sequence of the nascent peptide.

Allosteric regulation of the ribosomal A site revealed by molecular dynamics simulations.

The A site of the ribosome, which determines binding and orientation of a new amino acid residue for the peptidyl transferase reaction, was found to occupy two different conformational states upon the molecular dynamics (MD) simulations study of the 70S E. coli ribosome. One of the states, defined as "inactive", appeared in trajectories with E-tRNA, mutations A2531U and UU2492-3C, which are known to decrease the A site affinity to the tRNA.

A noncanonical binding site of chloramphenicol revealed via molecular dynamics simulations.

Chloramphenicol, an antibiotic belonging to the family of amphenicols, is an inhibitor of translation. On the basis of X-ray structural analysis of the binding of chloramphenicol to free bacterial ribosomes, the chloramphenicol action mechanism that consists in preventing the binding of aminoacyl-tRNA to the A-site of the large subunit of the ribosome was adopted. However, the known structures of chloramphenicol complexes with bacterial ribosomes poorly explain the results of the experiments on the chemical modification of 23S rRNA, the resistance to chloramphenicol caused by mutations in 23S rRNA and, which is particularly important, the selectivity of chloramphenicol in suppression of translation, depending on the amino acid sequence of the nascent peptide.

[The Dynamics of Primary Disability and Establishment of Losses of Healthy Life-Span of Able-Bodied Population of the Ryazan Oblast].

The problem of disability being a cause of decreasing of healthy life-span is actual especially nowadays when during last decade rate of increasing of primary disability of able-bodied population significantly advances mortality harming economics. The article considers dynamics of primary disability of population of the Ryazan oblast. The analysis of losses of healthy life-span of able-bodied population of the region caused by disability is implemented.

The Ribosome as an Allosterically Regulated Molecular Machine.

The ribosome as a complex molecular machine undergoes significant conformational rearrangements during the synthesis of polypeptide chains of proteins. In this review, information obtained using various experimental methods on the internal consistency of such rearrangements is discussed. It is demonstrated that allosteric regulation involves all the main stages of the operation of the ribosome and connects functional elements remote by tens and even hundreds of angstroms.

Tabby graphene: Dimensional magnetic crossover in fluorinated graphite.

Tabby is a pattern of short irregular stripes, usually related to domestic cats. We have produced Tabby patterns on graphene by attaching fluorine atoms running as monoatomic chains in crystallographic directions. Separated by non-fluorinated sp carbon ribbons, sp -hybridized carbon atoms bonded to zigzag fluorine chains produce sp -sp interfaces and spin-polarized edge states localized on both sides of the chains.