Although acute rejection (AR) has been shown to correlate with decreased long-term renal allograft survival, we have noted AR in recipients who subsequently had stable function for more than 5 years. We reviewed 109 renal graft recipients with a minimum of 1 year graft survival and follow-up of 5-8 years. Post-transplant sodium iothalamate clearances (IoCI) measured at 3 months and yearly thereafter were used to separate recipients into 2 groups.
View Article and Find Full Text PDFJ Microbiol Methods
October 2000
To determine the incidence of campylobacters in Northern Ireland pigs, ileal contents and anal swabs were taken shortly after death. Direct streaking onto Preston agar, and modified charcoal cefoperazone desoxycholate agar (mCCDA), were compared, as was enrichment in selective broths prior to streaking onto the corresponding solid medium. For anal swabs direct plating on mCCDA was most efficient, with 100% of samples positive, whilst for ileal contents enrichment in mCCD broth was best with 86% of samples positive.
View Article and Find Full Text PDFPaediatr Child Health
July 2000
An infant born at full term presented with tachypnea, tachycardia and fatigue with feedings. Blood gases revealed hypoventilation. A rigid bronchoscopy using the Karl-Storz telescopic system revealed severe tracheal stenosis.
View Article and Find Full Text PDFBackground: Two patients underwent cadaver transplantation with kidneys from a donor with a history of World Health Organization Class IV/V lupus nephritis, and we report their clinical and pathological outcome.
Methods: The donor had a diagnosis of lupus nephritis made by renal biopsy 5 years before donation. At the time of donation, a biopsy was performed on the donor and on one of the recipients at 2 months and 1 year after the transplant.
Proc Natl Acad Sci U S A
November 1999
Isotopic age determinations (40Ar/39Ar) and associated magnetic polarity stratigraphy for Casamayoran age fauna at Gran Barranca (Chubut, Argentina) indicate that the Barrancan "subage" of the Casamayoran South American Land Mammal "Age" is late Eocene, 18 to 20 million years younger than hitherto supposed. Correlations of the radioisotopically dated magnetic polarity stratigraphy at Gran Barranca with the Cenozoic geomagnetic polarity time scale indicate that Barrancan faunal levels at the Gran Barranca date to within the magnetochronologic interval from 35.34 to 36.
View Article and Find Full Text PDFSince the introduction of cyclosporine into clinical use, a major area of concern within the transplant community has been the fear of chronic nephrotoxicity. Although progressive renal damage does appear to occur in native kidneys of heart and liver transplant patients receiving cyclosporine, it has been our contention that its use is not a major cause of deterioration in renal allografts. We therefore undertook a study of 91 consecutive renal transplants performed over a three-year period with a minimum graft survival of 1 year and a follow-up of 7-9 years.
View Article and Find Full Text PDFObjective: To evaluate the effectiveness of initial treatment of children with acute immune thrombocytopenic purpura (ITP) with anti-D immune globulin (anti-D) or pooled IgG immune globulin (IVIg).
Study Design: The medical charts of 33 children diagnosed with acute ITP from May 1995 to October 1997 were reviewed. Patient data were eligible for analysis if, for the new diagnosis of acute ITP, the patient had received either anti-D at 45 to 50 microg/kg (WinRho SD, NABI) or IVIg at 0.
Pork liver (400) from bacon pigs (37 herds) obtained at six pork-processing in Northern Ireland were studied to assess the rate of contamination with Campylobacter spp. These animals average 95 to 100 kg live weight. Deep tissue areas were sampled immediately postevisceration and revealed that ca.
View Article and Find Full Text PDFSampling of lamb (n = 100) and beef (n = 100) carcasses in abattoirs in Northern Ireland produced no evidence of Campylobacter spp. contamination and when retail packs of beef (n = 50) and pork (n = 50) were sampled these were also apparently free of Campylobacter spp. However, 38% of retail packs of chicken pieces (n = 120), yielded Campylobacter spp.
View Article and Find Full Text PDFJ Res Natl Inst Stand Technol
October 1998
A high resolution VUV spectroscopic facility has been in use for several years at SURF II, the Synchrotron Ultraviolet Radiation Facility at the National Institute of Standards and Technology in Gaithersburg, Maryland. At this facility, a combination of three cylindrical mirrors is utilized to focus the light originating in the storage ring onto the horizontal entrance slit of the spectrometer. The spectrometer uses a 6.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
November 1997
The explanation of patterns in species richness ranks among the most important tasks of ecology. Current theories emphasize the interaction between historical and geographical factors affecting the size of the regional species pool and of locally acting processes such as competitive exclusion, disturbance, productivity, and seasonality. Local species richness, or alpha diversity, of plants and primary consumers has been claimed to peak in habitats of low and intermediate productivity, which, if true, has major implications for conservation.
View Article and Find Full Text PDFFermentation with Lactobacillus plantarum and Pediococcus cerevisiae was evaluated as a natural method of preservation for use in pork liver paté. Addition of both starter strains to a raw meat mix (10 CFU g) lowered the pH to 4.0 in 15 h at 30°C.
View Article and Find Full Text PDFNontuberculous mycobacteria are increasingly recognized as important pathogens in peritonitis associated with continuous ambulatory peritoneal dialysis (CAPD). Mycobacterium gordonae rarely causes human infection and is the least likely mycobacterium to produce clinical infection in CAPD patients. We describe a patient with persistent M.
View Article and Find Full Text PDFA comparison of the species richness and macroniche composition of diet, locomotor and body-size classes among 16 nonvolant mammalian faunas in tropical South America reveals numerous significant positive correlations with rainfall. In particular, significant and strong positive correlations with rainfall are found in 18 attributes, including the number of nonvolant mammal species, number of primate species, number of frugivores, primary consumers, arborealists, and the number of species between 100 g to 10 kg in body weight. Estimates of annual rainfall derived from least-squares and polynomial regressions and principal components analysis yield a modal estimate of between 1500 and 2000 mm annual rainfall for the Monkey Beds assemblage at La Venta.
View Article and Find Full Text PDFBased on a 10-mer primer (5'-CCTGTTAGCC-3'), a random amplified polymorphic DNA (RAPD) method for typing Campylobacter coli isolated from pigs was developed. The method proved effective with a high discrimination and good reproducibility. In contrast with serotyping no untypable strains were found out of a total of 269 isolates (veterinary, food and clinical) examined.
View Article and Find Full Text PDFFour hundred samples of processed animal protein were analysed for the presence of Salmonella using two impediometric methods. The direct method using both Easter and Gibson's, and Ogden's media, as defined in legislation applying throughout the UK, was less selective and sensitive, detecting 30% fewer positive samples than the indirect method using Rappaport-Vassiliadis broth. The indirect method detected 63 positive samples with a false positive rate of less than 10% and about 87% of positive samples were detected less than 12 h after being loaded onto the impedance equipment.
View Article and Find Full Text PDFThe feeding and reproductive performance of female lone star ticks (Amblyomma americanum (L.)) infesting guinea pigs on diets containing 15% fish oil (FO) or safflower oil (SO) were investigated. Replete ticks fed on FO-fed guinea pigs weighed approximately 30% less than those on the SO-fed guinea pigs.
View Article and Find Full Text PDFA survey of raw pork and a raw fermented pork sausage, chorizo, was undertaken in Mexico City to assess the hygienic quality of these two products on retail sale in a variety of outlets. Total bacterial counts and Enterobacteriaceae counts were determined and the samples were analyzed for the presence of Salmonella spp. Pork sold from refrigerated display cabinets in supermarkets and butchers' shops was of a poor microbial quality similar to that sold in street markets.
View Article and Find Full Text PDFArch Insect Biochem Physiol
February 1996
Tick saliva contains prostaglandins of the 2-series, believed to facilitate bloodmeal acquisition. Because ticks cannot synthesize the prostaglandin precursor, arachidonic acid, investigations were undertaken to study the uptake, incorporation, and distribution of arachidonic acid in the salivary glands of the lone star tick in vitro and in vivo. Uptake of [3H]arachidonate by isolated salivary glands was reduced in the presence of low concentrations of arachidonic or eicosapentaenoic acids, but much higher, non-physiological concentrations of oleic and linoleic acids were required to inhibit [3H]arachidonate uptake.
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