Publications by authors named "M van Waas"
Article Synopsis
- Studying native protein structures in crowded environments is challenging, especially in understanding their roles in diseases like Alzheimer's disease (AD).
- Researchers employed cryoelectron microscopy (cryo-EM) and the Build and Retrieve (BaR) method to analyze protein complexes in a rat model of AD, finding key structural characteristics linked to disease pathology.
- Insights from the study suggest that dysfunctions in these protein complexes are not limited to AD, but also relate to other conditions like cancer and various neurodegenerative disorders, highlighting potential new therapeutic approaches.
Deep mass spectrometry-based proteomic profiling of rare cell populations has been constrained by sample input requirements. Here, we present a protocol for droplet-based one-pot preparation for proteomic samples (DROPPS), an accessible low-input platform that generates high-fidelity proteomic profiles of 100-2,500 cells. We describe steps for depositing cellular material, cell lysis, and digesting proteins in the same microliter-droplet well.
View Article and Find Full Text PDFArticle Synopsis
- Urine shows how healthy someone is and gives clues about the health of the organs that create it, containing both secreted proteins and proteins in tiny bubbles called extracellular vesicles (EVs).
- Scientists studied the urine proteins of 190 men, including some with prostate cancer, and found a method to better collect prostate-related proteins from urine.
- The research shows that urine can help tell the difference between serious and less serious prostate issues and that these urine proteins stay pretty consistent over the years, which is useful for medical studies.
Biofluids contain molecules in circulation and from nearby organs that can be indicative of disease states. Characterizing the proteome of biofluids with DIA-MS is an emerging area of interest for biomarker discovery; yet, there is limited consensus on DIA-MS data analysis approaches for analyzing large numbers of biofluids. To evaluate various DIA-MS workflows, we collected urine from a clinically heterogeneous cohort of prostate cancer patients and acquired data in DDA and DIA scan modes.
View Article and Find Full Text PDFDeep proteomic profiling of rare cell populations has been constrained by sample input requirements. Here, we present DROPPS (droplet-based one-pot preparation for proteomic samples), an accessible low-input platform that generates high-fidelity proteomic profiles of 100-2,500 cells. By applying DROPPS within the mammary epithelium, we elucidated the connection between mitochondrial activity and clonogenicity, identifying CD36 as a marker of progenitor capacity in the basal cell compartment.
View Article and Find Full Text PDF