Cell kinetic analysis of a murine macrophage cell line.

For the present study, which was performed to find a reliable method suitable for determination of the cell kinetic parameters of a continuous cell line, use was made of the macrophage cell line J774.1. The doubling time of the cell population was approximately 27 h.

Morphological, cytochemical, functional, and proliferative characteristics of four murine macrophage-like cell lines.

The aim of the present study was to obtain objective data on the morphology and quantitative information about other characteristics of murine macrophage-like cell lines J774.1, PU5-1.8, WEHI-3, and P388-D1, and to compare the findings with those in resident and exudate macrophages collected directly from mice.

Comparison of the in vivo and in vitro proliferation of monoblasts, promonocytes, and the macrophage cell line J774.

Mononuclear phagocytes are localized in the bone marrow compartment (monoblasts, promonocytes and macrophages), the circulation (monocytes), and the tissues and serous cavities (macrophages). In vivo and in vitro studies done in murine bone marrow have shown that monoblasts and promonocytes are the most immature, dividing cells of the mononuclear phagocyte cell line; monocytes and resident macrophages do not divide. A very small percentage of the mononuclear phagocytes in the tissues, which are bone-marrow derived and have already the morphology of macrophages, divide once in vivo.