MinION™ Nanopore Sequencing of Skin Microbiome 16S and 16S-23S rRNA Gene Amplicons.

Human skin microbiome dysbiosis can have clinical consequences. Characterizing taxonomic composition of bacterial communities associated with skin disorders is important for dermatological advancement in both diagnosis and novel treatments. This study aims to analyze and improve the accuracy of taxonomic classification of skin bacteria with MinION™ nanopore sequencing using a defined skin mock community and a skin microbiome sample.

From Dysbiosis to Healthy Skin: Major Contributions of to Skin Homeostasis.

is the most abundant bacterium living in human, healthy and sebum-rich skin sites, such as the face and the back. This bacterium is adapted to this specific environment and therefore could have a major role in local skin homeostasis. To assess the role of this bacterium in healthy skin, this review focused on (i) the abundance of in the skin microbiome of healthy skin and skin disorders, (ii) its major contributions to human skin health, and (iii) skin commensals used as probiotics to alleviate skin disorders.

Olive mill sludge may reduce water contamination by 4-chloro-2-methylphenoxyacetic acid (MCPA) in non-flooding but enhance it in flooding rice cropping agroecosystems.

A field experiment covering three years was conducted to evaluate how composted olive mill sludge (OS) influenced MCPA's environmental fate in rice soils under six combinations of tillage and irrigation cultivation techniques: tillage and sprinkler irrigation without (TS) or with (TSOS) the addition of OS (80 Mg ha), no-tillage and sprinkler irrigation without (NTS) or with (NTSOS) OS, and tillage and continuous flooding without (TF) or with (TFOS) OS. The measurements made in the first and third years after OS application were taken to constitute the "direct" and "residual" effects, respectively. After OS amendment, K (partition coefficients) values in the direct year were lower by factors of 1.

How the environmental fate of clomazone in rice fields is influenced by amendment with olive-mill waste under different regimes of irrigation and tillage.

Background: Irrigation and tillage systems alone or in combination with organic amendments can strongly influence soil properties, which in turn may also modify the environmental fate of any pesticides applied. This study was aimed at determining how amendment with composted olive-mill waste (W) influenced leaching, sorption, and persistence of the herbicide clomazone in rice field soils under different tillage and irrigation management practices. The field trial conducted covered 3 years in succession, with six treatments: irrigation by sprinkler and conventional tillage without (ST) or with W application (80 Mg ha ) (STW), irrigation by sprinkler but no tillage (SNT), irrigation by sprinkler but no tillage with W application (SNTW), and continuous flooding irrigation and tillage without (FT) and with W application (FTW).

Gemcitabine, paclitaxel, and oxaliplatin (GEMPOX) in the treatment of relapsed/refractory intracranial nongerminomatous germ cell tumors.

Intracranial germ cell tumors (GCT) account for less than 5% of all central nervous system tumors in children in Western countries. Approximately 40% are nongerminomatous GCT (NGGCT). Despite correct treatment, 16% to 47% of the patients will relapse.