Plasmid-mediated colistin resistance among human clinical isolates: national surveillance in the Czech Republic.

The occurrence of colistin resistance has increased rapidly among around the world. We performed a national survey of plasmid-mediated colistin resistance in human clinical isolates through a retrospective analysis of samples from 2009 to 2017 and a prospective sampling in 2018-2020. The aim of this study was to identify and characterize isolates with genes from various regions of the Czech Republic using whole genome sequencing (WGS).

Characterization of the Complete Nucleotide Sequences of -Encoding Plasmids From Enterobacterales Isolates in Retailed Raw Meat Products From the Czech Republic.

The aim of our study was to determine complete nucleotide sequence of carrying plasmids from Enterobacterales isolates recovered from domestic and imported raw retailed meat and compare them with plasmids available at the GenBank sequence database. A set of 16 plasmids originating from ( = 13), ( = 2), and ( = 1) were analyzed. In our previous study, data from whole genome sequencing showed that gene was located on plasmids of different incompatibility groups (IncHI2, IncI2, and IncX4).

CTX-M-producing Escherichia coli in pigs from a Czech farm during production cycle.

We evaluated the prevalence and epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates in pigs during production cycle on a Czech farm with the history of previous use of ceftiofur. ESBL-producing E. coli isolates were obtained from rectal swabs from pigs of different age groups (suckling piglets, weaned piglets, growers and sows).

Dissemination and Comparison of Genetic Determinants of -Mediated Colistin Resistance in Enterobacteriaceae Retailed Raw Meat Products.

The global food chain may significantly promote the dissemination of bacteria resistant to antibiotics around the world. This study was aimed at determining the prevalence and genetic characteristics of Enterobacteriaceae with -mediated colistin (CT) resistance in retail meat of different origins. Bacteria of the Enterobacteriaceae family carrying the gene were detected in 21% (18/86) of the examined samples, especially in turkey meat and liver originating from EU and non-EU countries (19%) and in rabbit meat imported from China (2%).