Uterine leiomyosarcoma and pyometra in a dog.

A seven-year-old entire female, mixed-breed dog, weighing 19 kg, was presented with a 2-day history of abdominal distension, reduced appetite and general dullness. Abdominal palpation revealed a large mass. Radiography showed a large mass occupying the left mid-abdominal area and convoluted loops of tubular fluid opacity occupying the right mid-abdominal area.

Immunohistochemical expression of dog TERT in canine testicular tumours in relation to PCNA, ki67 and p53 expression.

The objective of the study was to determine the immunohistochemical expression of canine TERT in canine testicular tumours comparing two different antibodies for TERT, and to correlate them with well established markers specific to dividing cells such as PCNA and ki67, and with expression of the p53 tumour suppressor gene. The study included 36 cases of canine testicular tumours, which were categorized as 12 Sertoli Cell Tumours (SCT), 20 seminomas, 3 interstitial cell tumours and 1 mixed germ cell-sex cord stromal tumour (MT). Two antibodies for hTERT were examined; a highly specific TERT antibody, RCK-hTERT, was evaluated for the first time.

Telomerase reverse transcriptase (TERT) expression in canine mammary tissues: a specific marker for malignancy?

Background: Telomerase reverse transcriptase (TERT), the catalytic subunit of the enzyme telomerase, is expressed in virtually all human tumors. Telomerase activity has also been reported in the majority of canine tumors and dogTERT also correlates with the enzyme activity.

Materials And Methods: DogTERT expression in normal and malignant mammary tissues was investigated by immunohistochemistry and RT-PCR.

Canis familiaris telomerase reverse transcriptase undergoes alternative splicing.

The enzyme telomerase is essential for cell proliferation and tumorigenesis. Telomerase reverse transcriptase (TERT) represents the catalytic subunit of the enzyme. In humans, TERT expression is regulated by several different mechanisms, including alternative splicing.