[The effect of cyclosporine A on the development of Toxoplasma gondii in the muscles and brain. Ultrastructural study].

Presented are histological and electron microscopic findings of alterations in the muscles and brains of mice infected with T. gondii and suppressed with CyA during infection. Cya in the acute phase of infection promoted parasitemia.

[Cryptosporidium species in persons with HIV infection].

Oocysts of Cryptosporidium sp. were detected in the faeces of 65 subjects with the HIV virus, using the concentrating flotation method in a sugar solution and differential staining with methyl violet. In positive cases (a total of 16 subjects) continuous as well as intermittent excretion of oocysts in faeces was recorded, the longest excretion period being up to 15 months.

[Significance of the complement fixation reaction in the diagnosis of the acute phase of lymphatic toxoplasmosis].

In a group of 300 sera of subjects with clinically suspect nodular toxoplasmosis the authors tested by the IgM ELISA reaction in the reverse order the importance of the complement-fixation reaction for assessment of the acute stage of the infection. In view of the revealed prolonged detection of IgM antibodies the authors used quantitative evaluation of the reaction; they consider based on a probability pattern of antibody response coefficients of 10 and above important for differentiation of a recent infection. For the suggested method of evaluation it was confirmed that low CFR titres (1:8, 1:16, 1:32) imply usually (89.

Toxoplasmic antibodies in sera of HIV-infected persons.

The sera of 67 HIV-infected persons without clinical signs of Toxoplasma gondii infection and sera of 777 immunocompetent persons from three distinct regions of Czechoslovakia were examined for the presence of toxoplasmic antibodies using the complement-fixation test (CFT). Additionally Toxoplasma positive HIV+ individuals were re-examined for the presence of IgG and IgM toxoplasmic antibodies by ELISA methods. Results show that overall prevalence of toxoplasmic antibodies is not significantly greater in HIV-positive subjects (29.

[Induction of experimental pneumocystosis, detection and primary isolation of Pneumocystis carinii].

The described model of experimental pneumocystosis is based on the induction of natural latent infection of P. carinii in Wistar strain laboratory rats. As to pharmacological inducing agents immunosuppresive preparations such as Hydrocortisone sol.