Publications by authors named "M Z Sukhanova"
Fused in sarcoma (FUS) is involved in the formation of nuclear biomolecular condensates associated with poly(ADP-ribose) [PAR] synthesis catalyzed by a DNA damage sensor such as PARP1. Here, we studied FUS microphase separation induced by poly(ADP-ribosyl)ated PARP1 [PAR-PARP1] or its catalytic variants PARP1 and PARP1, respectively, synthesizing (short PAR)-PARP1 or (short hyperbranched PAR)-PARP1 using dynamic light scattering, fluorescence microscopy, turbidity assays, and atomic force microscopy. We observed that biologically relevant cations such as Mg, Ca, or Mn or polyamines (spermine or spermidine) were essential for the assembly of FUS with PAR-PARP1 and FUS with PAR-PARP1 in vitro.
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- - Current hematology studies often use immunodeficient mouse models to test patient-derived stem cells, but these models struggle with effectively incorporating cells from patients with myelodysplastic syndromes (MDS).
- - Researchers created a new bone marrow organoid model from induced pluripotent stem cells (iPSCs) that accurately mimics the human bone marrow environment, improving the engraftment of MDS patient stem cells and reflecting their unique disease traits.
- - The organoid model demonstrated that stem cells from both normal donors and MDS patients could proliferate and maintain their characteristics in a supportive microenvironment, revealing distinct differentiation patterns that correlate with MDS's clinical features and opening up possibilities for personalized treatments.
Deleterious germline variants constitute the most common inherited predisposition disorder linked to myeloid neoplasms (MNs). The role of DDX41 in hematopoiesis and how its germline and somatic mutations contribute to MNs remain unclear. Here we show that DDX41 is essential for erythropoiesis but dispensable for the development of other hematopoietic lineages.
View Article and Find Full Text PDFClonal hematopoiesis (CH) and clonal cytopenia of undetermined significance (CCUS) are recently recognized diagnostic entities that serve as independent risk factors for cardiovascular disease and myeloid malignancy. CH is an incidental finding, and evaluation of the incidence of CH/CCUS-associated mutations in solid tumor next-generation sequencing samples was undertaken to better understand the prevalence of mutations in this population. A retrospective review of clinical sequencing data for solid tumor malignancies diagnosed between February 2022 and April 2023 on next-generation sequencing data was performed.
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