Publications by authors named "M Yusupov"
Crystal structure of the GDP-bound GTPase Era from Staphylococcus aureus.
Biochem Biophys Res Commun
November 2024
GTPase Era from Staphylococcus aureus belongs to the TRAFAC superfamily of the TrmE-Era-EngA-EngB-Septin-like GTPases class and plays a significant role in the vital activity of this pathogenic microorganism as a maturation factor of the 30S ribosome subunit. However, the functions of this protein are not fully understood, making it a promising object for further study. Here, the 2.
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- Non-thermal plasma (NTP) shows potential as an anti-cancer therapy through both its ability to kill cancer cells and modify immune responses.
- The study uses simulations to analyze how NTP causes chemical changes in immune checkpoint proteins of natural killer (NK) cells, finding that these changes don't significantly impact their binding affinity to NK cell receptors.
- Additionally, NTP treatment leads to a quick decrease in certain inhibitory ligands for NK cells and an increase in activation ligands 24 hours later, highlighting its role in enhancing NK cell activity against cancer.
Here, we present the high-resolution structure of the Gallus gallus 80S ribosome obtained from cold-treated chicken embryos. The translationally inactive ribosome complex contains elongation factor eEF2 with GDP, SERPINE1 mRNA binding protein 1 (SERBP1) and deacylated tRNA in the P/E position, showing common features with complexes already described in mammals. Modeling of most expansion segments of G.
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- The YsxC protein in Staphylococcus aureus belongs to a family of GTP-binding proteins crucial for assembling the 50S ribosomal subunit, highlighting its role in the bacteria's life cycle.
- Recent studies suggest that targeting YsxC could lead to the development of new selective antimicrobials due to its unique structural features.
- Researchers cloned the ysxC gene, overexpressed it in E. coli, and successfully purified and crystallized the protein, achieving high-quality diffraction data for comparison with similar proteins.
One of the most critical steps of protein synthesis is coupled translocation of messenger RNA (mRNA) and transfer RNAs (tRNAs) required to advance the mRNA reading frame by one codon. In eukaryotes, translocation is accelerated and its fidelity is maintained by elongation factor 2 (eEF2). At present, only a few snapshots of eukaryotic ribosome translocation have been reported.
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