Unveiling immune cell response disparities in human primary cancer-associated fibroblasts between two- and three-dimensional cultures.

Cancer-associated fibroblasts (CAFs) play pivotal roles in solid tumor initiation, growth, and immune evasion. However, the optimal biomimetic modeling conditions remain elusive. In this study, we investigated the effects of 2D and 3D culturing conditions on human primary CAFs integrated into a modular tumor microenvironment (TME).

Use of a triple-coaxial system in coil embolization of a large bronchial artery aneurysm.

An 84-year-old man presented with a large bronchial artery aneurysm with a tortuous afferent artery. A triple-coaxial system was used to perform super-selective catheterization, consisting of a small microcatheter, a large microcatheter, and a 4-Fr catheter. The large microcatheter was successfully placed inside the aneurysm, and coil embolization was performed using large detachable coils to reduce the procedure cost.

Construction and Stability of All-in-One Adenovirus Vectors Simultaneously Expressing Four and Eight Multiplex Guide RNAs and Cas9 Nickase.

CRISPR/Cas9 technology is expected to offer novel genome editing-related therapies for various diseases. We previously showed that an adenovirus vector (AdV) possessing eight expression units of multiplex guide RNAs (gRNAs) was obtained with no deletion of these units. Here, we attempted to construct "all-in-one" AdVs possessing expression units of four and eight gRNAs with Cas9 nickase, although we expected obstacles to obtain complete all-in-one AdVs.