Publications by authors named "M Widschwendter"
The burden of uterine cancer is growing and, in the US and UK, mortality rates are poorest among black women. Early detection of these cancers is critical and poor performance of ultrasound in black women may contribute to adverse outcomes. Limited data on this topic are available from Africa.
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- The study examines the reliability of the Illumina Methylation array platform in measuring DNA methylation by evaluating the consistency of repeated measures using both type I and type II Infinium probes.
- A new method is proposed to identify unreliable probes by using dynamic thresholds for mean intensity (MI) and unreliability scores based on simulations that factor in technical noise.
- Validation across multiple datasets indicates that probes with low MI tend to show higher variability in β values, and an R package is introduced to help researchers calculate MI and unreliability scores for better data analysis.
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- Biomarkers of aging (BOA) are special measurements that can help scientists understand how old someone is on a biological level and how this changes with treatments.
- Recently, many new BOA have been discovered that could really help people live healthier lives as they age, but there are some problems getting these ideas into actual medical practice.
- Experts found six main challenges that are stopping BOA from being used more widely and suggested ways to make them better, such as ensuring they are easy to access and useful for everyone.
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- Mifepristone (MIF) is a synthetic hormone used for pharmacological abortions and treating certain medical conditions, and the study aimed to create a reliable method to measure MIF and its metabolite (MET) in plasma and tissue samples from mice.
- The researchers developed a high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) method that efficiently extracts and quantifies these substances, with quick run times and high sensitivity.
- The method demonstrated effective separation and detection limits that could potentially be adapted for human medical research.
Purpose: The purpose of this study was to evaluate RB1 expression and survival across ovarian carcinoma histotypes and how co-occurrence of BRCA1 or BRCA2 (BRCA) alterations and RB1 loss influences survival in tubo-ovarian high-grade serous carcinoma (HGSC).
Experimental Design: RB1 protein expression was classified by immunohistochemistry in ovarian carcinomas of 7,436 patients from the Ovarian Tumor Tissue Analysis consortium. We examined RB1 expression and germline BRCA status in a subset of 1,134 HGSC, and related genotype to overall survival (OS), tumor-infiltrating CD8+ lymphocytes, and transcriptomic subtypes.