Publications by authors named "M Wagenknecht"

Article Synopsis
  • - The study focuses on improving the production of antibody fragments (Fabs) in E. coli, specifically analyzing the responses of two strains, BL21(DE3) and HMS174(DE3), during the expression of different Fabs using RNA sequencing.
  • - Findings showed that the production of Fabx negatively impacted cell growth more than FTN2, with RNA-seq revealing stress responses related to protein aggregation and cell envelope integrity, alongside nutrient depletion during the cultivation process.
  • - The research identified ribosome stalling as a key factor contributing to low yields of Fabx and demonstrated that modifying the amino acid sequence could enhance production, along with unexpected differences in gene expression responses between the two E. coli strains.
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This document was created based on the need to standardize the psychological examination procedure prior to bariatric surgery. A valuable inspiration was the recommendations issued by the American Society for Metabolic and Bariatric Surgery. Bariatric or metabolic surgery has an undeniable positive effect in the treatment of obesity, in terms of improving somatic diseases, psychological disorders and psychosocial functioning.

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Antibody fragments such as Fab's require the formation of disulfide bonds to achieve a proper folding state. During their recombinant, periplasmic expression in , oxidative folding is mediated by the DsbA/DsbB system in concert with ubiquinone. Thereby, overexpression of Fab's is linked to the respiratory chain, which is not only immensely important for the cell's energy household but also known as a major source of reactive oxygen species.

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Obesity is a metabolic disorder conditioned by several factors with the individual genetic proneness to accumulation of body fat with a positive energetic balance. If such definition describes the essential nature of obesity aptly, the treatment thereof ought to be the realm of somatic medicine and somatically oriented physicians, which is, unfortunately, frequently the case. Yet, not only being a disorder concerning improper body composition, but also a difference in cognitive processes and emotions of the obese, obesity needs to be considered in a more complex manner.

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Overweight and obesity prevalence in middle aged subjects in the Czech Republic is more than 50 per cent, obesity is found in around 26 per cent of population. Obesity management is a long-term and time-consuming process. Early start of the treatment can prevent continuous weight gain and development of co-morbidities.

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