Point spread function estimation with computed wavefronts for deconvolution of hyperspectral imaging data.

Hyperspectral imaging (HSI) systems acquire images with spectral information over a wide range of wavelengths but are often affected by chromatic and other optical aberrations that degrade image quality. Deconvolution algorithms can improve the spatial resolution of HSI systems, yet retrieving the point spread function (PSF) is a crucial and challenging step. To address this challenge, we have developed a method for PSF estimation in HSI systems based on computed wavefronts.

A label-free real-time method for measuring glucose uptake kinetics in yeast.

Glucose uptake assays commonly rely on the isotope-labeled sugar, which is associated with radioactive waste and exposure of the experimenter to radiation. Here, we show that the rapid decrease of the cytosolic pH after a glucose pulse to starved Saccharomyces cerevisiae cells is dependent on the rate of sugar uptake and can be used to determine the kinetic parameters of sugar transporters. The pH-sensitive green fluorescent protein variant pHluorin is employed as a genetically encoded biosensor to measure the rate of acidification as a proxy of transport velocity in real time.

Artificial ER-Derived Vesicles as Synthetic Organelles for Compartmentalization of Biochemical Pathways.

Compartmentalization in membrane-surrounded organelles has the potential to overcome obstacles associated with the engineering of metabolic pathways, such as unwanted side reactions, accumulation of toxic intermediates, drain of intermediates out of the cell, and long diffusion distances. Strategies utilizing natural organelles suffer from the presence of endogenous pathways. In our approach, we make use of endoplasmic reticulum-derived vesicles loaded with enzymes of a metabolic pathway ("metabolic vesicles").

[Hybrid esophagectomy with intraoperative hyperspectral imaging : Video article].

The technique of hybrid esophagectomy with systematic 2‑field lymphadenectomy for esophageal cancer showed a significant reduction in postoperative morbidity in a recently published prospective randomized study. This video publication presents the abdominothoracic hybrid procedure with (i) laparoscopic gastrolysis and ischemic conditioning of the stomach and (ii) 2-stage transthoracic esophagectomy with gastric pull-up, intrathoracic gastric tube formation and anastomosis. Intraoperative hyperspectral imaging (HSI) during the thoracic part of the operation is used for identification of the ideally perfused anastomotic region.