Public Health.

Background: The Impact of Intensive Treatment of Systolic Blood Pressure on Brain Perfusion, Amyloid and Tau in Older Adults (IPAT) is an ongoing randomized control trial (RCT) for prevention of Alzheimer's disease (AD) (NCT05331144). The primary outcome of the trial is to assess the impact of intensive lowering of high systolic blood pressure (SBP) on the accumulation of brain amyloid in older adults for a study duration of 2 years. Study recruitment started on October 25 of 2022 and will end in September of 2025.

A modular system for programming multistep activation of endogenous genes in stem cells.

Unlabelled: Although genomes encode instructions for mammalian cell differentiation with rich syntactic relationships, existing methods for genetically programming cells have modest capabilities for stepwise regulation of genes. Here, we developed a sequential genetic system that enables transcriptional activation of endogenous genes in a preprogrammed, stepwise manner. The system relies on the removal of an RNA polymerase III termination signal to induce both the transcriptional activation and the DNA endonuclease activities of a Cas9-VPR protein to effect stepwise progression through cascades of gene activation events.

Understanding Support for Municipal Political Parties: Evidence from Canada.

The province of Ontario, Canada, has a longstanding history of non-partisanship in municipal elections. In this distinctive context, we report results on citizen attitudes toward municipal partisanship using a survey of eligible voters in Canada's most populous province. Using a mixed-methods approach, we focus on three interrelated research questions.