Publications by authors named "M Vorlickova"
Article Synopsis
- - G-quadruplexes (G4s) in RNA are becoming important targets for cancer and disease therapies, with GG motifs being more common than GGG motifs in RNA sequences.
- - Recent findings indicate that DNA cannot make stable two-tetrad G4 structures, raising questions about the stability of RNA GG motifs and their potential regulatory functions.
- - This study reveals that RNA GG motifs typically don't form stable intramolecular G4s by themselves; instead, they need to dimerize to create stable structures, which may play a role in RNA aggregation during cellular stress.
Human ARMC6 binds in vitro to both cancer genes and telomeric RNA, favoring G-quadruplex structure recognition.
Biochim Biophys Acta Gene Regul Mech
September 2024
Article Synopsis
- Armadillo repeat-containing proteins (ARMCs) are important for various cellular functions like adhesion, signaling, and regulating cellular structures, with ARMC6 being significant in primates but its specific roles were previously unclear.
- Recent experiments show that ARMC6 binds to DNA promoter sequences of cancer-related genes and to telomeric RNA, suggesting involvement in chromosomal maintenance via recognition of G-quadruplex structures.
- Overexpression of ARMC6 in human cell lines leads to altered expression of oncogenic and telomerase-related genes, highlighting its potential role in linking gene regulation with telomeric chromatin rearrangement.
Cytosine-rich DNA regions can form four-stranded structures based on hemi-protonated C.C+ pairs, called i-motifs (iMs). Using CD, UV absorption, NMR spectroscopy, and DSC calorimetry, we show that model (CnT3)3Cn (Cn) sequences adopt iM under neutral or slightly alkaline conditions for n > 3.
View Article and Find Full Text PDFObjectives: Non-communicable diseases are estimated to account for 90 % of total deaths and 19 % of premature deaths in Slovakia. Major preventable risk factors of premature mortality are overweight, obesity and alcohol consumption.
Background: Screening of risk factors related to alcoholic and nonalcoholic fatty liver diseases (AFLD and NAFLD, respectively) in Slovak outpatients with liver disease.
We have identified seven putative guanine quadruplexes (G4) in the RNA genome of tick-borne encephalitis virus (TBEV), a flavivirus causing thousands of human infections and numerous deaths every year. The formation of G4s was confirmed by biophysical methods on synthetic oligonucleotides derived from the predicted TBEV sequences. TBEV-5, located at the NS4b/NS5 boundary and conserved among all known flaviviruses, was tested along with its mutated variants for interactions with a panel of known G4 ligands, for the ability to affect RNA synthesis by the flaviviral RNA-dependent RNA polymerase (RdRp) and for effects on TBEV replication fitness in cells.
View Article and Find Full Text PDF