Immobilisation of β-galactosidase within a lipid sponge phase: structure, stability and kinetics characterisation.

In the formulation of an active enzyme enclosed in a matrix for controlled delivery, it is a challenge to achieve a high protein load and to ensure high activity of the protein. For the first time to our knowledge, we report the use of a highly swollen lipid sponge (L) phase for encapsulation of the large active enzyme, β-galactosidase (β-gal, 238 kDa). This enzyme has large relevance for applications in, e.

Encapsulation of Aspartic Protease in Nonlamellar Lipid Liquid Crystalline Phases.

Encapsulation of proteins within lipid inverse bicontinuous cubic phases (Q) has been widely studied for many applications, such as protein crystallization or drug delivery of proteins for food and pharmaceutical purposes. However, the use of the lipid sponge (L) phase for encapsulation of proteins has not yet been well explored. Here, we have employed a lipid system that forms highly swollen sponge phases to entrap aspartic protease (34 kDa), an enzyme used for food processing, e.

On the Molecular Interactions in Lipid Bilayer-Water Assemblies of Different Curvatures.

This work concerns the importance of intermolecular interactions present in aqueous lipid assembly systems depending on the type of aggregates they form. We have studied aqueous mixtures of diglycerol monooleate, Capmul glycerol monoleate (GMO-50) and polyoxyethylene (20) sorbitan monooleate (Polysorbate 80, P80) using small-angle X-ray scattering (SAXS) measurements to reveal the structure of liquid crystalline phases. On the basis of the SAXS data, a phase diagram was constructed.

Interfacial properties of lipid sponge-like nanoparticles and the role of stabilizer on particle structure and surface interactions.

The advantage of using nonlamellar lipid liquid crystalline phases has been demonstrated in many applications, such as drug delivery, protein encapsulation and crystallisation. We have recently reported that a mixture of mono- and diglycerides is able to form sponge-like nanoparticles (L3-NPs) with large enough aqueous pores to encapsulate macromolecules such as proteins. Here we use small angle neutron scattering (SANS) to reveal morphology, structural and chemical composition of these polysorbate 80 (P80) stabilized sponge phase nanoparticles, not previously known.

Large retropharyngeal haematoma. Airway management with Airtraq® laryngoscope.

Retropharyngeal haematoma is a life-threatening clinical situation that can lead to a potential obstruction of the upper airway and requires rapid diagnosis. Clinicaly, it can be presented in different ways, depending on its size and growing speed. The first measure is to protect and manage the airway: in most cases this is a difficult airway situation.