Differential editing efficiencies in cereal crops: a comparative analysis of tRNA and ribozyme multiplexed guide delivery.

Cereal transformation and gene editing can be a complex and costly undertaking. It is therefore important to validate and understand the performance of the components to achieve high rates of transformation and gene editing. Here, we have made a direct comparison of different CRISPR/Cas9 guide systems to target the genome in three cereal species.

Effect of elevated incubation temperatures on learning and brain anatomy of hatchling and juvenile lizards.

Global warming is a major threat to reptiles because temperature strongly affects their development. High incubation temperatures reduce hatchling body size and physiological performance; however, its effects on brain development and learning abilities are less well understood. In particular, it remains unclear if the effects of elevated temperatures on learning are restricted to hatchlings or instead will persist later in life.

Lifetime history of head injury is associated with reduced perivascular space number in acute mild traumatic brain injury.

Traumatic brain injury impairs function of the glymphatic system, a perivascular network involved in waste clearance. Enlarged perivascular spaces visible on MRI are an emerging biomarker of glymphatic function. This study characterized enlarged perivascular spaces in acute head injury with 7 T MRI.

Pain, inconvenience and blame: defining work-related injuries in the veterinary workplace.

Background: The veterinary workplace carries a high risk of staff accidents and injuries, yet there is scant research exploring it in comparison with other comparable fields, such as human medicine.

Aims: To understand how veterinary professionals define injuries and to understand what injuries they do, or do not, deem reportable.

Methods: A cross-sectional survey comprising demographic questions and open-text questions was shared with veterinary practice staff across the UK.

Single nucleotide polymorphisms in the cannabinoid CB receptor: Molecular pharmacology and disease associations.

Preclinical evidence implicating cannabinoid receptor 2 (CB) in various diseases has led researchers to question whether CB genetics influence aetiology or progression. Associations between conditions and genetic loci are often studied via single nucleotide polymorphism (SNP) prevalence in case versus control populations. In the CNR2 coding exon, ~36 SNPs have high overall population prevalence (minor allele frequencies [MAF] ~37%), including non-synonymous SNP (ns-SNP) rs2501432 encoding CB 63Q/R.