Pecularities of Candida yeast identification by mass spectrometric analysis (MALDI-ToF MS).

To carry the comparative analysis of sample preparation methods for the most effective identification of Candida yeast by mass spectrometric analysis. 265 strains of yeast and yeast-like fungi isolated from the sputum of patients with pneumonia were investigated. The selected strains were identified by conventional methods (cultural, morphological, tinctorial, enzymatic properties) and MALDI-ToF MS using the Autoflex speed III Bruker Daltonics mass spectrometer (Germany) and Flex Control software.

[Comparative analysis of parameters of humoral and cell specific immunity in individuals immunized with a live tularemia vaccine].

Aim: Comparative analysis of parameters of humoral and cell immunity in individuals, vaccinated against tularemia.

Materials And Methods: Sera and blood samples of 258 immunized individuals were studied by indirect hemagglutination and leukocytolis with tularin reaction.

Results: 73% of the examined individuals had both specific antibodies and positive values of cell immunity.

[Features of resistance formation to beta-lactam antibiotics in Francisella tularensis subsp. mediasiatica].

Aim: Determination of penicillin resistance features in tularemia causative agents of the mediasiatica subspecies, stability evaluation of differences in strains of various taxa and development of a rapid method of F. tularensis intraspecies differentiation.

Materials And Methods: Beta-lactamase activity was determined in 30 strains of Francisella genus bacteria by quantitative iodometric method.

[The phosphatase activity in representatives of the genus Francisella].

The comparative study of phosphatase activity in the representatives of the genus Francisella--F. tularensis, F. novicida, F.

[A rapid method for assessing the virulence of Francisella tularensis in vitro].

A rapid and simple method for the in vitro evaluation of the virulence of F. tularensis is proposed. The optimal parameters--the concentrations of bacteria and the substrate (phenolphthalein phosphate), the time incubation in the serum--have been developed and special conditions for strains of different subspecies have been selected.