Determining the level of stromal and epithelial cells activity in normal and hyperplastic endometrium of late reproductive and perimenopausal women.

Hyperplastic processes of the endometrium (HPE) are a group of benign endometrial and stromal cells that have undergone altered growth. This study aimed to investigate the potential role of hypoxia (as indicated by Hif-1α) and apoptosis markers (p53 and BCL-2) in the development of hyperplastic processes of the endometrium (HPE). Results showed that endometrial cells with atypical hyperplasia had increased levels of Hif-1ɑ, which indicates the presence of endometrial hypoxia and may trigger pathological manifestations.

Effect of immunization with polyvinylpyrrolidone on the counts of stromal precursor cells in bone marrow and spleen of CBA and CBA/N mice and cytokine gene expression in primary cultures of these cells.

Injection of polyvinylpyrrolidone (synthetic type 2 T-independent antigen) stimulated the efficiency of clone-forming efficiency and the content of stromal precursor cells in CBA mice in the femoral bone marrow (almost 3-fold) and in the spleen (by 1.7 times) with the peak within 24 h and normalization by day 3 after immunization. The expression of IL-6, IL-8, and TNF-α genes in bone marrow and spleen cultures from immunized animals appeared on day 1 and disappeared on day 3.

[The length-force dependence of vascular smooth muscles and the nitric oxide system in a chronic deficiency of mesostriatal dopamine].

This study investigated the influence of the chronic cerebral dopamine deficiency after 6-hydroxydopamine lesions of dopaminergic mesostriatal system and NO pathways on the length-tension relation in vascular smooth muscles to their distension. Experiments were performed on isolated strips of rat portal vein. The results indicate that the attenuated contraction responses and the increased stiffness of vascular smooth muscles to the distension in a chronic mesostriatal dopamine deficiency.

[The suppression of c-fos protein expression in the islands of Calleja in damage to the mesostriatal dopaminergic system of the brain].

We found activation c-fos protein in the NO synthase-containing neurons within the islands of Calleja (96.46 +/- 16.03 fos-immunoreactive nuclei per 50 mm section) in control and suppression of this activation after unilateral 6-hydroxydopamine lesions of dopaminergic mesostriatal system of brain (14.