Publications by authors named "M V Semenova"
Article Synopsis
- The study tested four different mouse strains (inbred Balb/c, C57Bl/6, hybrid DBA/2*C57Bl/6, and outbred ICR) to determine the effectiveness of four culture media on embryo development.
- Results showed that embryo development varied significantly based on both the strain and the culture medium used, with inbred and outbred strains being more sensitive to suboptimal conditions than hybrids.
- It concluded that optimal culture media vary by strain, and a combination of different strains is necessary for accurate testing, as relying on single strains can lead to erroneous results.
Macro and micro enhancers of the 8-anilino-1-naphthalenesulfonate (ANS) fluorescence. Is ANS indeed a hydrophobic probe?
Spectrochim Acta A Mol Biomol Spectrosc
December 2024
A study on the absorption and fluorescence properties of the 8-anilino-1-naphthalenesulfonate (ANS) fluorescent probe was performed in order to (i) verify the validity of its classification as hydrophobic probe and (ii) to assess the reliability of the interpretation of the ANS fluorescence enhancement upon protein binding as the evidence for the existence of hydrophobic binding sites on the protein molecules. We observed an enhancement of the ANS fluorescence in hydrophilic media: DMSO, polyethylene glycol (PEG400) and glycerol to the values characteristic of ANS complexes with globular proteins, and all ANS fluorescence characteristics (except anisotropy) in PEG400 and in complex with bovine serum albumin are identical. We observed an increase in the ANS fluorescence with a nonzero anisotropy in an aqueous medium in the presence of an amphiphilic cetyltrimethylammonium cation as a result of the formation of the 1:1 complex with ANS.
View Article and Find Full Text PDFCytochrome (CytC), a one-electron carrier, transfers electrons from complex to cytochrome oxidase (CcO) in the electron-transport chain. Electrostatic interaction with the partners, complex and CcO, is ensured by a lysine cluster near the heme forming the Universal Binding Site (UBS). We constructed three mutant variants of mitochondrial CytC with one (2Mut), four (5Mut), and five (8Mut) Lys->Glu substitutions in the UBS and some compensating Glu->Lys substitutions at the periphery of the UBS for charge compensation.
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- Essential polyunsaturated fatty acids (PUFAs), particularly n-3 and n-6, are important for maintaining health, but the specific lipids that provide benefits are not clearly identified.
- This study focused on the effects of various liposomal diets on liver phospholipid composition in mice, analyzing the fatty acid profiles using chromatography-mass spectrometry after feeding them different liposomal mixtures over three months.
- The results showed that one specific liposomal complex, containing phosphatidylcholine, clove bud essential oil, fish oil, and sodium caseinate, significantly increased n-3 fatty acids and decreased n-6 fatty acids in liver phospholipids, suggesting potential anti-inflammatory effects and a lower risk of certain diseases
Neuroglobin (Ngb) is a cytosolic heme protein that plays an important role in protecting cells from apoptosis through interaction with oxidized cytochrome (Cyt ) released from mitochondria. The interaction of reduced Ngb and oxidized Cyt is accompanied by electron transfer between them and the reduction in Cyt . Despite the growing number of studies on Ngb, the mechanism of interaction between Ngb and Cyt is still unclear.
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