Publications by authors named "M V SABANTSEV"
Front Bioeng Biotechnol
November 2024
Article Synopsis
- Adeno-associated virus (AAV)-based gene therapy aims to treat hereditary disorders by replacing faulty genes, but the challenge is that many of these genes are larger than AAV's 5 kilobase capacity.
- Researchers are exploring strategies like truncated gene versions and intein-based protein trans splicing to restore full gene functionality, focusing on the effectiveness of different inteins from the DnaE group.
- The NpuDnaE variant has shown promising results, achieving 80% GFP assembly in HEK293 cells, indicating potential for higher efficiency in gene therapy applications.
