Publications by authors named "M V Rajam"
Article Synopsis
- Tomato plants expressing dsRNA targeting Fusarium oxysporum f. sp. lycopersici (Fol) have been developed, enhancing their resistance to the fungal infection and significantly reducing disease symptoms.
- Through RNA interference (RNAi) technology, transgenic tomatoes were created to silence specific Fol genes associated with disease, leading to a substantial decrease in Fol colonization and improved plant health.
- The study confirms that host-induced gene silencing (HIGS) effectively provides durable resistance against Fusarium wilt in tomatoes, suggesting a viable biofungicide strategy without harming the plant’s overall photosynthetic function.
Fusarium oxysporum f. sp. lycopersici (Fol) is majorly responsible for causing vascular wilt disease in tomato by blocking transpirational pull, thereby interfering and suppressing overall host immune response.
View Article and Find Full Text PDFRNA interference and crop protection against biotic stresses.
Physiol Mol Biol Plants
October 2021
RNA interference (RNAi) is a universal phenomenon of RNA silencing or gene silencing with broader implications in important physiological and developmental processes of most eukaryotes, including plants. Small RNA (sRNA) are the critical drivers of the RNAi machinery that ensures down-regulation of the target genes in a homology-dependent manner and includes small-interfering RNAs (siRNAs) and micro RNAs (miRNAs). Plant researchers across the globe have exploited the powerful technique of RNAi to execute targeted suppression of desired genes in important crop plants, with an intent to improve crop protection against pathogens and pests for sustainable crop production.
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- Maruca vitrata is a significant pest affecting legume crops globally, and understanding its molecular mechanisms can help in developing control strategies.
- Researchers evaluated the stability of eight candidate reference genes using quantitative real-time PCR to determine the most consistent ones for gene expression studies in M. vitrata across varying conditions.
- The findings indicate that RP49 and RPL13 are the most reliable reference genes for different developmental stages, while RP49, RPL24, and GAPDH were ideal for studies based on diet and sex, enhancing the accuracy of future gene functional studies in this pest.
Unlabelled: In the present study, we have explored the potential of the RNAi mediated silencing of genes encoding peroxisomal biogenesis factor and β-1,3-glucanosyltransferase in f. sp. lyco to confer resistance to Fusarium wilt in transgenic tomato plants.
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