[Changes in immunologic phenotype of cells in hairy cell leukemia].

A case of hairy-cell leukemia (HCL) has been described in which the cells of the pathological clone in the course of time lost their surface immunoglobulins and retained membrane markers associated with the T-lymphoid series. It has been suggested that this change in the immunological phenotype of the cells may lead to an erroneous diagnosis of T-cell HCL.

[Electron microscopy morphometry of cells of patients with different immunologic subvariants of acute lymphoblastic leukemia].

By the electron-microscopic morphometry it is determined that the cells of acute lymphoblastic leukemia (ALL) with synonymous immunological phenotype also have the same submicroscopic structure and are characterized by definite morphometric features which distinguish them from lymphoblasts of other subvariants. These results indicate that the electron-microscopic morphometry is one of the criteria for precise identification of leukemic cells. Besides, the similarity of the morphometric features of leukemic cells of the concrete lineage with the synonymous differentiation status confirm their lineage fidelity.

[Electrophoretic mobility of the cells of non-T, non-B acute lymphoblastic leukemia].

In order to ascertain the possibility of the use of electrophoretic mobility (EPM) as one of the criterion for delicate identification of the leukemic cell, a study was made of the EPM of blast cells having an approximately equal status of differentiation. A study was also made of lymphoblasts of the most frequently occurring variant of acute lymphoblastic leukemia, namely of the non-T, non-B variant. It has been established that the given cells are marked by the similar values of the EPM, which greatly exceed the analogous characteristics of normal mature B lymphocytes, being lower as compared with the EPM of T lymphocytes.

[Ultrastructural characteristics of immunologic subvariants of acute T-cell lymphoblastic leukemia].

Ultrastructural comparison was conducted between different immunological subvariants of T-cell acute lymphoblastic leukemia (T-ALL). Using the panel of monoclonal antibodies the cases of T-ALL were categorized into the following subvariants (early-T-, mature-T- and mature, activated-T-cell ALL). It has been shown that cells of these subvariants differ from each other in their ultrastructure and at the same time they reflect the submicroscopic features of corresponding normal counterparts.