[Features of the metabolism of nitric oxide in normal state and inflammation].

Brief analysis of the metabolism of nitric oxide in living cells in normal state and pathology and also the analysis of the causes, that hampered the progress of these studies, were carried out. It was established that most of physiological fluids, including blood, normally contain nitrite and non-thiolate nitroso compounds in concentration less than 100 nM. Literature data from different researchers on the normal range of nitrite concentration in plasma of healthy people from several hundreds of nM to several microM is probably the result of low selectivity of the methods used.

Mechanism of therapeutic effect of low-intensity infrared laser radiation.

The effect of infrared low-intensity laser irradiation on functional activity of blood polymorphonuclear leukocytes was studied in vitro. A dose-dependent priming of polymorphonuclear leukocytes induced by infrared low-intensity laser irradiation was demonstrated. Similar effects were also observed in the presence of the photosensitizer photosense.

Priming of phagocytes by cytokines and water-soluble products of lipid peroxidation.

It is well known that during certain pathological processes phagocytes acquire the ability to generate activated oxygen species during phagocytosis. The priming of phagocytes by cytokines and water-soluble products of lipid peroxidation (LPO) is described. Preincubation of human polymorphonuclear leukocytes (PMNL) with the water-soluble products of LPO or oxidised liposomes for 15-20 min at 37 degrees C enhanced their functional activity when they were stimulated by opsonised zymosan or latex particles.

[Changes in the superoxide dismutase activity during stimulation of polymorphonuclear leukocytes of peripheral blood].

The functional activity of the peripheral blood polymorphonuclear leukocytes (PML) was investigated by using the method of latex-stimulated luminol-dependent chemiluminescence (CL). The CL-intensity of PML taken from patients with acute myocardial infarction (MI) was found to be 20 times higher than that of normal individuals (NI). The change in activity of endogenous antioxidative enzyme systems may account for alteration of PML CL-parameters.

[Characteristics of immune complexes in myocardial infarct].

Nonspecific circulating immune complexes (CIC), different in size and stability, are detectable in the blood sera of patients with myocardial infarction; changed concentration of these complexes appears to be a reflection of the defense response of the body, aimed at homeostasis maintenance. No relationship between cardiac glycosides and CIC levels was revealed in patients with myocardial infarction. A reduction of the level of 'large' CICs by days 21-30 of the condition was observed in the patients treated with anticoagulants from the first day of the disease.