[The characteristics of formation of antibodies to xeno- and endobiotics in women with inherent malformation of fetus].

The study revealed high levels of antibodies class A and G to benzopyrene and antibodies class G to estradiol and progesterone in women with pathology of fetus development. The most informative indicators of high risk of development of reproductive pathology are established The recommendations concerning the application of antibodies to benzopyrene, estradiol and progesterone in diagnostics of inherent malformations of fetus are provided.

[Preparation and characterization of the recombinant protein containing immunomimetic peptide of benzo[a]pyrene].

Two recombinant plasmids were constructed. The first plasmid contained the hybrid gene composed of immunomimetic peptide of benzo[a]pyrene, of the protein pIII of bacteriophage M13 and of cellulose binding domain encoding sequences. The second plasmid contained the hybrid gene composed of the signal peptide of the protein pIII of bacteriophage M13, of immunomimetic peptide of benzo[a]pyrene, of the protein pill of bacteriophage M13 and of cellulose binding domain sequences.

[Features of interaction of monoclonal antibody B2 with polycyclicaromatic hydrocarbons and peptide-mimotope of benzo[a]pyrene].

To define of possible mechanism of monoclonal antibody B2 interaction with haptens and peptide-mimotope of benzo[a]pyrene Fab-fragment structure was modeled. Antibody active centre structure was defined using docking with a number of different polyclic aromatic hydrocarbons. It was shown that active center of monoclonal antibody B2 composed of two binding pockets.

[New approach to synthesis of peptide immunomimetic of benzo[a]pyrene].

A novel approach to discovery of the peptide with an internal immunological image of carcinogen is suggested in this work. The hybridomas producing monoclonal antibodies (mAb) B2 against benzo[a]pyrene and benz[a]antracene were prepared. Polyclonal Ab against benzo[a]pyrene (Bp) and benz[a]antracene (Ba), antracene, chrysene, and pyrene were also prepared.

[A new immunoassay of human antibodies to chemical carcinogens].

The study was undertaken to optimize the well known immunoassay of antibodies to chemical carcinogens, designed by J. Chagnaud et al., and examine the specific features of the generation of antibodies (Ab) to benzo[a]pyrene (BP) in patients with lung cancer.