[Formation of resting cells in microbial suspensions undergoing autolysis].

Under experimentally selected conditions favoring spontaneous or induced autolysis of cell suspensions, the asporogenous bacteria Escherichia coli and Methylococcus capsulatus, the bacilli Bacillus cereus (under conditions of suppressed sporulation), and the yeast Saccharomyces cerevisiae were shown to be capable of forming cystlike resting cells. Their number was influenced by (1) cell density in the suspensions; (2) the presence of Ca2+ ions in nutrient-limited medium; (3) pH of medium; and (4) autolysis rate, dependent on the concentration of oleic acid (a chemical analogue of the autolysis-inducing d2 factor) introduced into the cell suspensions.

[Formation of a resting form of Bacillus cereus and Micrococcus luteus].

Under certain cultivation conditions, the bacteria Bacillus cereus and Micrococcus luteus form cystlike refractive cells (up to 60% of the total number) that retain viability over a long time, are metabolically inactive and thermotolerant and possess specific ultrastructure. These properties allow them to be attributed to a new type of resting forms of microorganisms.

[Treatment of fatty hepatosis in diabetics].

A ten-day course of karsil (silimarin, legalon) or silibor therapy improved the absorption (as indicated by ueviridin test) and oxidative-antitoxic (as shown by antipyrine test) hepatocyte functions in patients with non-insulin-dependent diabetes mellitus with hepatosis. A similar ten-day course of Liv-52 did not change the studied liver parameters. Inducto-thermal exposure of the splenic area (a 20 min session daily for 5 days) improved the absorption and antitoxic functions of the liver in diabetics.

[Tyrosol--the autoregulatory d1 factor of the yeast Saccharomyces cerevisiae].

The autoregulatory d1 factor of the yeast, Saccharomyces cerevisiae, that induces the transition of vegetative cells into refractory resting forms, has been isolated from the cell-free culture medium as an individual crystalline compound. It has been shown to be 2-(4-hydroxyphenyl)ethane-1-ol which is also known as tyrosol. When added to the producer culture at 5-15 microM concentration, tyrosol stimulated the endogenous respiration of cells, but inhibited at 20-80 microM concentration.