Fe and Mn complexes based on 2,4,6-tris(2-pyridyl)-triazine: synthesis, structures, magnetic and biological properties.

Reaction of the polypyridyl ligand 2,4,6-tris(2-pyridyl)--triazine (tpt) with mono- and polynuclear Fe(iii) or Mn(ii) precursors, specifically tri- or hexanuclear Fe(iii) pivalates (piv), [MnO(piv)(Hpiv)] and Mn(ii) isobutyrate (ib), in various solvents and under various reaction conditions showcase the ligand's surprising coordination characteristics. The reactions result in mononuclear [Fe(tpt)(tptH)][FeCl]·2(thf)·0.23(HO) (1), [Fe(piv)(tpt)Cl] (2), [Fe(tpt)Cl]·2(HO) (3a), dinuclear [Fe O(tpt)Cl] (3), and heptanuclear [Fe O(piv)(tpt-O)]·A [A = MeCN (4a) or 4(dioxane) (4b)] and [Fe O(piv)(tpt-O)(i-PrO)(i-PrOH)]·0.

A new reliable reference gene UBA52 for quantitative real-time polymerase chain reaction studies in pyloric cecal tissues of the starfish Asterias rubens.

The starfish Asterias rubens is one of the most abundant echinoderm species in the White, Barents, North, and Baltic Seas. This species is an important component of marine ecosystems and a model object for certain biological studies, in particular those requiring quantitative estimation of gene expression. As a rule, expression at the transcriptional level is estimated by real-time qPCR using the ΔΔCt method, which allows the comparison of the copy number of target gene transcripts in samples with unknown mRNA/cDNA concentration.

[Differential expression of genes that encode glycolysis enzymes in kidney and lung cancer in humans].

Glycolysis is a main catabolic pathway of glucose metabolism, accompanied by ATP synthesis. More than 30 enzymes are involved in glycolysis, and genes that encode them can be considered housekeeping genes due to the high conservatism and evolutionary antiquity of the process. We studied the expression of these genes in kidney papillary cancer and planocellular lung cancer via the bioinformatic analysis of transcriptome database and method of quantitative real time PCR.

[Dimeric bisberzimidazoles: cytotoxicity and effects on DNA methylation in normal and cancer human cells].

Cancer cells are characterized by the hypermethylation of promoter regions of tumor suppressor genes. DNA methyltransferase inhibitors cause re-activation of these genes that allows considering DNA methyltransferases as targets for anticancer therapy. As it was previously shown by us, dimeric bisbenzimidazoles, DB(n), differing in length of the oligomethylene linker between the two bisbenzimidazole fragments (n--number of methylene groups in linker) effectively inhibit the methylation of DNA duplexes by murine methyltransferase Dnmt3a.