A follow-up study of urinary bladder patients tested for tumour-related lymphocyte-mediated cytotoxicity.

A total of 143 patients with transitional cell carcinoma of the urinary bladder were tested for lymphocyte-mediated cytotoxicity against the bladder carcinoma cell line T24. Some of the patients were also tested against MANO (another cell line of transitional cell bladder carcinoma origin), HCV29 (from bladder epithelium, probably transformed in vitro) and/or HT29 (from a colon adenocarcinoma). The patients were divided into a high- or a low-responder group for each cell line.

Plasma membrane-associated antigens on tumor cells derived from transitional-cell carcinoma of the human urinary bladder. II. Identification at the molecular level of plasma membrane-associated antigens.

The surface proteins of seven human cell lines (three bladder carcinomas (TCC), two normal urothelial lines, one colon carcinoma, and one malignant melanoma) were labelled with 125I by the glucose oxidase-lactoperoxidase technique. Plasma membranes of the cells were isolated and analysed by sodium dodecyl sulphate electrophoresis (SDS-PAGE). When analysed under reducing conditions by staining with protein stain, approximately 45 distinct membrane polypeptides were detected in all membrane preparations.

Membrane-associated antigens on tumor cells from transitional-cell carcinoma of the human urinary bladder. I. Immunological characterization by xenogeneic antisera.

An antiserum was raised in rabbits by immunization with a human tumor cell line, T-24, derived from transitional cell carcinoma (TCC) of the urinary bladder. The specificity of the IgG fraction was assessed by antibody-dependent cellular cytotoxicity (ADCC), using purified blood lymphocytes from healthy human donors as effector cells and seven human cell lines as target cells (T-24 and two additional TCC-lines, two normal urothelial lines, one colon carcinoma and one malignant melanoma). The IgG induced strong lysis of all seven target cell types.

Lymphocyte-mediated lysis of tumor cells in vitro (ADCC), induced by serum antibodies from patients with urinary bladder carcinoma or from controls.

IgG fractions from serum of patients with transitional-cell carcinoma of the urinary bladder (TCC), patients with carcinoma of the prostate (CC) and healthy donors (HD) were tested for their capacity to induce antibody-dependent lymphocyte-mediated cytotoxicity (ADCC) to tumor cells in vitro. Lymphocytes from healthy donors were selected for low natural cytotoxicity to the target cells from established cell lines of TCC or other origins. IgG was prepared by adsorption of serum to Sepharose-bound protein A from Staphylococcus aureus and subsequent acid elution.