The value of bacterial metagenomic analysis in post-surgical examination of gallstones.

Gallstone disease is one of the most common causes of hospitalization for gastrointestinal diseases in the world. Recent studies have examined the presence of bacteria in the formation of stones. Our main goal was to determine the overall composition of gallstone microflora.

Clinical use of NGS data from the targeted gene panel for mitochondrial diseases screening.

Background And Objective: Mitochondrial diseases are a frequent cause of inherited genetic disorders caused by mutations in both the mitochondrial and nuclear human genome. The new technique of high-throughput sequencing, which is used more and more frequently around the world, is most often focused on nuclear DNA. In some cases, such data after proper IT processing could also allow to determine alterations in mtDNA genome.

Usefulness of droplet digital PCR and Sanger sequencing for detection of FGFR3 mutation in bladder cancer.

Objective: The purpose of our research was to determine the usefulness of different methods for detecting Y373C mutation of gene FGFR3.

Patients And Methods Total: 138 primary bladder cancer patients (71cases G1 and 67 cases G2-G3) were included in the study. Tumor tissue and urine samples were collected and kept frozen until the isolation of DNA.

Urine miRNA as a potential biomarker for bladder cancer detection - a meta-analysis.

Introduction: White light cystoscopy (WLC), often supported by urine cytology, is considered the 'goldstandard' in the diagnosis and follow-up of bladder cancer (BCa). In recent years, urine microRNA (miRNA) tests have been performed for the detection of bladder cancer.

Material And Methods: A systematic review of the PubMed platform was performed by searching for articles in which miRNA in the urine was used for the detection of BCa.

Detection of bladder cancer in urine sediments by a hypermethylation panel of selected tumor suppressor genes.

Background: Promoter hypermethylation can be a useful biomarker for early detection and prognosis of bladder cancer, monitoring response to treatment and complement classical diagnostic procedures.

Objective: The molecular test was performed on DNA from bladder cancer cells in voided urine samples, tumor tissue DNA and normal control DNAs. We aimed to assess the diagnostic potential of epigenetic changes in urine DNA from bladder cancer cases at various clinico-pathological stages of the disease.