Over the past decades, the focus of brain research has expanded from using strictly controlled stimuli towards understanding brain functioning in complex naturalistic contexts. Interest has increased in measuring brain processes in natural interaction, including classrooms, theatres, concerts and museums to understand the brain functions in the real world. Here, we examined how watching a live dance performance with music in a real-world dance performance setting engages the brains of the spectators.
View Article and Find Full Text PDFPurpose: Our objective was to elucidate host dependent factors of disease severity in invasive group A Streptococcal disease (iGAS) using transcriptome profiling of iGAS cases of varying degrees of severity at different timepoints. To our knowledge there are no previous transcriptome studies in iGAS patients.
Methods: We recruited iGAS cases from June 2018 to July 2020.
Here, we present a modification of single-cell tagged reverse transcription protocol to study gene expression on a single-cell level or with limited RNA input. We describe different enzymes for reverse transcription and cDNA amplification, modified lysis buffer, and additional clean-up steps before cDNA amplification. We also detail an optimized single-cell RNA sequencing method for handpicked single cells, or tens to hundreds of cells, as input material to study mammalian preimplantation development.
View Article and Find Full Text PDFStudies in the neuroscience of music gained momentum in the 1990s as an integrated part of the well-controlled experimental research tradition. However, during the past two decades, these studies have moved toward more naturalistic, ecologically valid paradigms. Here, I introduce this move in three frameworks: (i) sound stimulation and empirical paradigms, (ii) study participants, and (iii) methods and contexts of data acquisition.
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