Publications by authors named "M Takizawa"

Single-cell genomics technologies have accelerated our understanding of cell-state heterogeneity in diverse contexts. Although single-cell RNA sequencing identifies rare populations that express specific marker transcript combinations, traditional flow sorting requires cell surface markers with high-fidelity antibodies, limiting our ability to interrogate these populations. In addition, many single-cell studies require the isolation of nuclei from tissue, eliminating the ability to enrich learned rare cell states based on extranuclear protein markers.

View Article and Find Full Text PDF
Article Synopsis
  • The study evaluates a new scanning method called Us-IRHF that enhances image quality while speeding up the acquisition time for MRCP images without extending the scanning duration.
  • Using slit phantoms and a clinical trial with 41 patients, the researchers compared Us-IRHF to conventional scanning methods and found that Us-IRHF produced superior image quality and maintained adequate contrast ratios.
  • The results suggest that Us-IRHF is a promising technique for clinical use, offering improved visualization of biliary and pancreatic ducts without compromising on scan time.
View Article and Find Full Text PDF
Article Synopsis
  • Recent advancements in genetic manipulation have made it possible to create specific deletions in mitochondrial DNA (mtDNA) in human cells, which can help study diseases linked to these genetic changes.
  • A method involving co-expression of end-joining machinery and targeted endonucleases was developed, showcasing effectiveness by generating clonal cell lines with a significant mtDNA deletion and varying levels of heteroplasmy.
  • Research showed that when mtDNA deletion reached around 75%, it led to severe cellular dysfunction and identified distinct nuclear gene expression changes in response to mtDNA deletions, suggesting insights for potential therapies.
View Article and Find Full Text PDF

We measure the complete set of angular coefficients J_{i} for exclusive B[over ¯]→D^{*}ℓν[over ¯]_{ℓ} decays (ℓ=e, μ). Our analysis uses the full 711  fb^{-1} Belle dataset with hadronic tag-side reconstruction. The results allow us to extract the form factors describing the B[over ¯]→D^{*} transition and the Cabibbo-Kobayashi-Maskawa matrix element |V_{cb}|.

View Article and Find Full Text PDF

Nanoscale-patterned Au surfaces are promising for a wide range of applications from bio/chemical sensors to high-performance electrodes. However, pattern formation using conventional resist-based methods is complex, expensive, and environmentally unfriendly. Herein, we report a novel approach for pattern formation on Au surfaces using solid-state electrochemical treatment with polymer electrolyte membrane (PEM) stamps.

View Article and Find Full Text PDF