Rapid, fully automated measurement of plasma homocyst(e)ine with the Abbott IMx analyzer.

We have developed a totally automated fluorescence polarization immunoassay for homocyst(e)ine with no pretreatment or chromatographic steps. Comparison with four well-established chromatographic methods yielded r values ranging from 0.980 to 0.

Hydrophilic fluorescein derivatives: useful reagents for liposome immunolytic assays.

Hydrophilic derivatives of fluorescein containing hydroxyalkyl substituents were synthesized and encapsulated within liposomes. The fluorophores showed significantly more retention with time than did fluorescein, carboxyfluorescein, or calcein. Unlike calcein, the fluorophores are minimally susceptible to fluorescence quenching by Co2+.

4'-[Aminomethyl]fluorescein and its N-alkyl derivatives: useful reagents in immunodiagnostic techniques.

A new class of fluorescein derivatives with chemically reactive amino and N-alkylamino "arms" in the 4'-position were synthesized and their utility in the development of fluorescence polarization immunoassays (FPIA) for cortisol and estriol was evaluated. The positioning of the arm in one of the phenolic rings introduced chirality due to hindered rotation and led to rotational isomers. These were separable when brought into a chiral environment, i.

A revised structure of fluorescein mercuric acetate.

With the help of NMR spectroscopy the structure assignment of fluorescein mercuric acetate is corrected to 4',5'-bis(acetoxymercury)fluorescein.

Allyl derivatives of zearalenone.

The Claisen rearrangement of 4-O-allylzearalenone led to only one principal product, which was identified as 3-allylzearalenone with the aid of photochemical isomerization and PMR spectroscopy. This general method is useful for distinguishing 3-substituted zearalenone isomers from 5-substituted isomers. Hydrogenation of 3-allylzearalenone gave 3-(1-propyl)zearalanone.