Publications by authors named "M Sunbul"
Super-Resolved Protein Imaging Using Bifunctional Light-Up Aptamers.
Angew Chem Int Ed Engl
December 2024
Efficient labeling methods for protein visualization with minimal tag size and appropriate photophysical properties are required for single-molecule localization microscopy (SMLM), providing insights into the organization and interactions of biomolecules in cells at the molecular level. Among the fluorescent light-up aptamers (FLAPs) originally developed for RNA imaging, RhoBAST stands out due to its remarkable brightness, photostability, fluorogenicity, and rapid exchange kinetics, enabling super-resolved imaging with high localization precision. Here, we expand the applicability of RhoBAST to protein imaging by fusing it to protein-binding aptamers.
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- Observing individual RNA molecules helps scientists understand how they work and interact within cells, but previous methods like fluorescent light-up aptamers (FLAPs) faced challenges with brightness and stability.
- The new biRhoBAST avidity-based FLAP improves this by enabling high-quality single-molecule RNA imaging in both live and fixed cells due to its superior brightness and photostability.
- This text outlines detailed protocols for using biRhoBAST to track mRNA dynamics and includes methods for validating these imaging experiments through techniques like smFISH and colocalization studies.
Article Synopsis
- The study investigates the wall thickness of the inferior vena cava (IVC) in patients with Behçet's disease (BD) compared to healthy controls, revealing that BD patients have significantly thicker IVC walls.
- Results showed that IVC wall thickness is associated with mucocutaneous and major organ involvement, indicating a correlation between vascular issues and BD symptoms.
- The findings suggest that venous wall inflammation in BD affects not only lower extremity veins but also larger venous structures like the IVC, pointing to a widespread vascular inflammation in this disease.
Background: Myocardial infarction (MI) is increasing at a younger age. Growth differentiation factor-15 (GDF-15) has been implicated in several key mechanisms of atherogenesis. Arterial stiffness parameters, including pulse wave velocity (PWV) and the augmentation index (AIx), can indicate the presence or progression of atherosclerosis.
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