The ABF4-bHLH28-COMT5 module regulates melatonin synthesis and root development for drought tolerance in citrus.

Melatonin significantly influences the regulation of plant growth, development, and stress tolerance. However, the regulatory mechanisms underlying melatonin accumulation for drought tolerance in citrus are not fully understood. In this study, we first demonstrated that application of exogenous melatonin resulted in better drought tolerance by reducing water loss and maintaining redox homeostasis.

Single-cell and chromatin accessibility profiling reveals regulatory programs of pathogenic Th2 cells in allergic asthma.

Lung pathogenic T helper type 2 (pTh2) cells are important in mediating allergic asthma, but fundamental questions remain regarding their heterogeneity and epigenetic regulation. Here we investigate immune regulation in allergic asthma by single-cell RNA sequencing in mice challenged with house dust mite, in the presence and absence of histone deacetylase 1 (HDAC1) function. Our analyses indicate two distinct highly proinflammatory subsets of lung pTh2 cells and pinpoint thymic stromal lymphopoietin (TSLP) and Tumour Necrosis Factor Receptor Superfamily (TNFRSF) members as important drivers to generate pTh2 cells in vitro.

Comparison of hook plates vs. locking plates for Neer type IIB fractures of lateral end clavicle: A systematic review.

Purpose: Surgical management of the lateral end of clavicle fractures has been a challenge for orthopedic surgeons considering the high rate of non-union. There has been no right and wrong answer to these types of fractures and many methods discussed in the literature, but the 2 most used bony procedures are hook plate and locking plate with or without the use of supplementary soft tissue procedures. The available evidence, in this case, is scarce with questionable reliability.

ProtCB-bind: Protein-carbohydrate binding site prediction using an ensemble of classifiers.

Proteins and carbohydrates are fundamental biomolecules that play crucial roles in life processes. The interactions between these molecules are essential for various biological functions, including immune response, cell activation, and energy storage. Therefore, understanding and identifying protein-carbohydrate binding regions is of significant importance.

Diagnosis of Indian Big Four and monocled Cobra snakebites in envenomed plasma using smartphone-based digital imaging colourimetry method.

Background: Venomous or dry bites can result from snake envenomation. Therefore, developing a detection test for venomous snakebites in envenomed patients can prevented from unnecessary antivenom therapy for dry bites, thereby, saving them from adverse effects and cost of antivenom therapy.

Methodology: This study demonstrates a method for the diagnosis of medically significant 'Big Four' Indian snake venoms (Naja naja, Bungarus caeruleus, Daboia russelii, Echis carinatus) in the plasma of experimentally envenomed animals (envenomed under laboratory conditions).