Electrophoretic mobility of blood lymphocytes stored at -196 degrees.

To determine the influence of low temperature (-196 degrees C) during preservation upon the electrophoretic mobility (EM) of lymphocytes ten healthy donors were examined. Lymphocytes were isolated from venous blood by Ficoll-Uropolinum (Polfa) density gradient centrifugation and iron powder technique was used to eliminate macrophages. EM of lymphocytes was analyzed on Parmoquant 2 computer system (Carl-Zeiss, Jena) before and ten days after cryopreservation.

Electrophoretic mobility of leukocytes in leukemia patients.

Lymphocytes isolated from the blood of healthy individuals (analyzed on Parmoquant-2 apparatus) showed mean electrophoretic mobility (EM) of 1.00 +/- 0.05 um sec-1 V-1 cm and the histogram appeared to be an asymmetric curve - sum of B and T lymphocytes subpopulation.

Cooperative randomized studies on the treatment of adult acute leukemia in Poland. A comparison of two remission induction regimes and two maintenance regimes for AML.

In a prospective study 100 AML cases were randomized in two groups and underwent remission induction treatment in 4 departments with "Ara-C c. inf. from the first to the eight day plus Adr.