Histo-FISH protocol to detect bacterial compositions and biofilms formation in vivo.

The study of biofilm function in vivo in various niches of the gastrointestinal tract (GIT) is rather limited. It is more frequently used in in vitro approaches, as an alternative to the studies focused on formation mechanisms and function of biofilms, which do not represent the actual in vivo complexity of microbial structures. Additionally, in vitro tests can sometimes lead to unreliable results.

Analysis of biofilm formation by intestinal lactobacilli.

In this study, the biofilm-forming potential of intestinal Lactobacillus reuteri strains under different culture conditions was characterized by microtiter plate biofilm assays. Moreover, the spatial organization of exogenously applied L. reuteri L2/6 (a pig isolate) at specific locations in gastrointestinal tract of monoassociated mice was investigated by fluorescence in situ hybridization.

[Therapeutic compliance and its pharmaceutical aspects. Basic research].

In present-day drug therapy, the level of therapeutic compliance is a marked professional problem. Its consequences are a challenge for providers of health care and a concern for pharmacists as subjects working in immediate contact with the general public of patients. The present paper reports an evaluation of the level of compliance and analyses its components.