Publications by authors named "M Sinniah"
Rapid diagnostic tests (RDTs) can detect anti-malaria antibodies in human blood. As they can detect parasite infection at the low parasite density, they are useful in endemic areas where light infection and/or re-infection of parasites are common. Thus, malaria antibody tests can be used for screening bloods in blood banks to prevent transfusion-transmitted malaria (TTM), an emerging problem in malaria endemic areas.
View Article and Find Full Text PDFHigh levels of anti-dengue IgM or IgG can be detected using numerous rapid diagnostic tests (RDTs). However, the sensitivity and specificity of these tests are reduced by changes in envelope glycoprotein antigenicity that inevitably occur in limited expression systems. A novel RDT was designed to enhance diagnostic sensitivity.
View Article and Find Full Text PDFBackground: Dengue is a mosquito-borne disease that causes a public health problem in tropical and subtropical countries. Current immunological diagnostics based on IgM and/or nonstructural protein 1 (NS1) antigen are limited for acute dengue infection due to low sensitivity and accuracy.
Objectives: This study aimed to develop a one-step multiplex real-time RT-PCR assay showing higher sensitivity and accuracy than previous approaches.
This is a retrospective study of the gastrointestinal symptoms, signs and laboratory parameters in adult dengue patients admitted to Kuala Lumpur Hospital from 1st December 2004 to 31st December 2004. Clinical and laboratory parameters that may predict the need for intensive care were investigated. Six hundred sixty-six patients with clinical and biochemical features consistent with dengue infection were identified.
View Article and Find Full Text PDFArticle Synopsis
- The Nipah virus nucleocapsid protein (NiV-N) was successfully expressed in E. coli and purified for use as a diagnostic antigen.
- Two types of ELISA tests, one for IgG and one for IgM, were developed using this NiV-N protein to detect infections in humans and swine.
- The new ELISA tests displayed high sensitivity and specificity, effectively identifying more cases than traditional methods, making them reliable and cost-effective for widespread use, particularly in developing countries.