Publications by authors named "M Sila-Asna"
Article Synopsis
- Human corneal cells are in high demand for replacement due to limited availability, prompting research into alternatives.
- The study successfully developed corneal epithelial-like cells from human skin-derived precursor cells (hSKPs) using a combination of three growth factors: EGF, KGF, and HGF.
- Induced hSKPs exhibited characteristics of corneal epithelial cells, confirmed by specific markers and gene expression, indicating potential for using skin-derived cells in treating corneal disorders in future clinical settings.
Article Synopsis
- Vasculogenic mimicry (VM) allows cancer cells to imitate blood vessel formation, particularly in hepatocellular carcinoma (HCC), potentially linked to metastasis.
- The study found that only poorly-differentiated HCC cells (SK-Hep-1) could form VM in vitro, while well-differentiated cells (HepG2) could not.
- Hepatocyte growth factor (HGF) was able to induce VM in HepG2 cells along with changes like epithelial-mesenchymal transition and increased stemness gene expression, suggesting that the influence of stemness genes on VM varies with cell differentiation status.
Article Synopsis
- Parathyroid hormone (PTH) is a promising new treatment for osteoporosis, as it boosts the differentiation of osteoblasts from skin-derived precursor cells in lab cultures.
- The study compared two groups: one receiving only an osteogenic induction medium (OM) and the other receiving OM plus PTH (OM + PTH), with results showing that the OM + PTH group had stronger indicators of osteoblast maturation.
- Key findings included increased expression of osteoblast-specific genes and proteins (like osteocalcin and bone sialoprotein) in the OM + PTH group, suggesting PTH may enhance bone formation and maturation in osteoporosis therapy.
The role of vitamin K2 on osteoblastic functions by using stem cell model.
J Med Assoc Thai
September 2009
Vitamin K2 (MK4) functions were investigated by using the induced skin cell into osteoblast compared with the control media. The real time PCR measured gene expression in both cultures at the fourth, seventh, fourteenth, twenty first, twenty eighth, thirty fifth and forty second days of culture. The gene expressions of osteocalcin, osteonectin, osteopontin, bone sialoprotein, CBFA1, Interleukin-6, Estrogen receptors and collagen type) were monitored by real time PCR.
View Article and Find Full Text PDFBackground: The invasion of cancer cells is critical for metastasis. The effects of Eclipta prostrata, a Thai medicinal plant, on invasion, migration and adhesion of cancer cells were investigated and the anti-angiogenic activity in vivo was evaluated.
Materials And Methods: In vitro invasion and migration assays were performed in modified Boyden chambers.