Production and functional analysis of a phage displayed scFv recombinant antibody targeting EGFR/HER2 dimerization domain.

Background: Tumor cells exploit epidermal growth factor receptor (EGFR) family to develop resistance against therapeutic antibodies, such as Herceptin. Upon ligand binding, dimerization between EGFR and HER2 is one of the most important causes of treatment failure in breast cancer and other cancers expressing EGFR and HER2. The aim of this study was to develop and evaluate the function of a human recombinant single-chain variable fragment (scFv) antibody against the dimerization domain of EGFR to inhibit its interaction with other members of the epidermal growth factor receptor family, especially HER2.

Translation, Cross-Cultural Adaptation, Reliability, and Validity of the Persian Version of the Neck Outcome Score Questionnaire in Iranian Patients With Nonspecific Neck Pain.

Objective: The primary purpose of this study was to translate and culturally adapt the neck outcome score (NOOS) questionnaire into Persian language and investigate its reliability and validity.

Methods: The NOOS questionnaire was translated into Persian language and culturally adapted according to American Academy of Orthopaedic Surgeons guidelines. One hundred four patients with nonspecific neck pain were requested to complete the Persian version of the NOOS questionnaire, 36-item short form survey (SF-36), and neck disability index (NDI) questionnaire.

Design, synthesis, and evaluation of novel substituted imidazo[1,2-c]quinazoline derivatives as potential α-glucosidase inhibitors with bioactivity and molecular docking insights.

α-Glucosidase inhibitors are important in the treatment of type 2 diabetes by regulating blood glucose levels and reducing carbohydrate absorption. The present study focuses on identifying new inhibitors bearing imidazo[1,2-c]quinazoline backbone through multi-step synthesis. The inhibitory potencies of the novel derivatives were tested against Saccharomyces cerevisiae α-glucosidase, revealing IC values ranging from 50.

Design, synthesis, and cytotoxic activity of 2-amino-1,4-naphthoquinone-benzamide derivatives as apoptosis inducers.

A new series of 2-amino-1,4-naphthoquinone-benzamides 5a-n was designed based on previously reported potent cytotoxic agents. These compounds were synthesized from the reaction of 1,4-naphthoquinone, 4-aminobenzoic acid, and appropriate amine derivatives in good yields. Cytotoxic activities of the target compounds 5a-n were evaluated against three cancer cell lines MDA-MB-231, SUIT-2, and HT-29 by MTT assay and the obtained in vitro data.