The purpose of the present study was to investigate the mechanism of action of our newly developed green sanitizer formulation comprising a natural phenolic compound, gallic acid (GA), strengthened by the Generally Recognized as Safe (GRAS) materials hydrogen peroxide (HO) and DL-lactic acid (LA). Combining 8 mM GA with 1 mM HO resulted in an abundant generation of reactive oxygen species (ROS) and a bactericidal effect towards Gram-negative (, , and ) and Gram-positive () bacteria (4 to 8 log CFU mL reduction). However, the exposure to this dual formulation (DF) caused only a modest 0.
View Article and Find Full Text PDFNitrogen fixation is a key process that sustains life on Earth. Nitrogenase is the sole enzyme capable of fixing nitrogen under ambient conditions. Extensive research efforts have been dedicated to elucidating the enzyme mechanism and its artificial activation through high applied voltage, photochemistry, or strong reducing agents.
View Article and Find Full Text PDFDespite regulatory and technological measures, edible sprouts are still often involved in foodborne illness and are considered a high-risk food. The present study explored the potential of spore-forming isolates to mitigate and contamination of alfalfa sprouts. Food-derived strains were screened for antagonistic activity against serovar Typhimurium SL1344 (STm) and enteropathogenic O55:H7.
View Article and Find Full Text PDFPulcherrimin is an iron-binding reddish pigment produced by various bacterial and yeast species. In the soil bacterium Bacillus subtilis, this pigment is synthesized intracellularly as the colorless pulcherriminic acid by using two molecules of tRNA-charged leucine as the substrate; pulcherriminic acid molecules are then secreted and bind to ferric iron extracellularly to form the red-colored pigment pulcherrimin. The biological importance of pulcherrimin is not well understood.
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