Diet therapy abates mutant APC and KRas effects by reshaping plasma membrane cholesterol nanodomains.

Cholesterol-enriched plasma membrane domains are known to serve as signaling platforms in a diverse array of cellular processes. However, the link between cholesterol homeostasis and mutant APC-KRas-associated colorectal tumorigenesis remains to be established. Thus, we investigated the impact of Apc-Kras on (i) colonocyte plasma membrane cholesterol homeostasis, order, and receptor nanoclustering, (ii) colonocyte cell proliferation, and (iii) whether these effects are modulated by select membrane active dietaries (MADs).

Graphical Dirichlet Process for Clustering Non-Exchangeable Grouped Data.

We consider the problem of clustering grouped data with possibly non-exchangeable groups whose dependencies can be characterized by a known directed acyclic graph. To allow the sharing of clusters among the non-exchangeable groups, we propose a Bayesian nonparametric approach, termed graphical Dirichlet process, that jointly models the dependent group-specific random measures by assuming each random measure to be distributed as a Dirichlet process whose concentration parameter and base probability measure depend on those of its parent groups. The resulting joint stochastic process respects the Markov property of the directed acyclic graph that links the groups.

Freytag's dramatic structure: Reflections on designing and delivering impactful trainings.

While many student leadership educators facilitate trainings, research has shown most never actually learn how to design and deliver impactful experiences. This article provides a proven framework for consistently designing and delivering high-impact, high-engagement, and high-enjoyment trainings that leverage experiential learning and reflective dialogue. The "Training Story" method is based on the dramatic structure introduced by German playwright Gustav Freytag (1895).

Evolution During Bottle Ageing of Wines Macerated with Toasted Vine-Shoots and Micro-Oxygenation.

The effects of SEGs ("Shoot from vines-Enological-Granule") on winemaking within the same variety are well established. However, the interaction of different SEG varieties combined with micro-oxygenation (MOX) and its subsequent evolution in the bottle has not been investigated to date. In this work, Tempranillo wines were treated with two doses of SEGs from Tempranillo and Cabernet Sauvignon (12 and 24 g/L) and subjected to two fixed MOX doses (LOTR, 6.